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Anti-AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [ST48-09]

重组兔单抗 | 货号: ET1609-51


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  • 概述

  • 产品描述 The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
  • 产品名称 Anti-AKT1/2/3 Recombinant Rabbit Monoclonal Antibody [ST48-09]
  • 分子量 Predicted band size: 56 kDa
  • 种属反应性 Human,Mouse,Rat
  • 验证应用 WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr
  • 抗体类型 重组兔单抗
  • 免疫原 Recombinant protein within human AKT3 aa 300-479.
  • 偶联 Non-conjugated
  • RRID AB_2940862
  • 性能

  • 形态 Liquid
  • 浓度 1 mg/mL.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • 亚型 IgG
  • 纯化方式 Protein A affinity purified.
  • 亚细胞定位 Cell membrane, Cytoplasm, Membrane, Nucleus.
  • 数据链接 SwissProt: P31749 Human
    SwissProt: P31751 Human
    SwissProt: Q9Y243 Human
    SwissProt: P31750 Mouse
    SwissProt: Q60823 Mouse
    SwissProt: Q9WUA6 Mouse
    SwissProt: P47196 Rat
    SwissProt: P47197 Rat
    SwissProt: Q63484 Rat
  • 其它名称
    • AKT antibody
    • AKT1 antibody
    • AKT1 kinase antibody
  • 应用

    WB: 1:5,000
    IF-Cell: 1:50-1:200
    IF-Tissue: 1:50-1:200
    IHC-P: 1:50-1:200
    FC: 1:50-1:100
    IP: Use at an assay dependent concentration.
    IHC-Fr: 1:100

  • 背景文献

  • 1. Buendia, I. et al. 2015. The Melatonin-N,N-Dibenzyl(N-methyl)amine Hybrid ITH91/IQM157 Affords Neuroprotection in an in Vitro Alzheimer's Model via Hemo-oxygenase-1 Induction. ACS chemical neuroscience. 6: 288-96.

    2. Siendones, E. et al. 2014. Membrane-bound CYB5R3 is a common effector of nutritional and oxidative stress response through FOXO3a and Nrf2. Antioxidants & redox signaling. 21: 1708-25.
  • Publishing research using Anti-AKT1/2/3 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

  • PMID:
    37031824
    Mechanisms of action underlying the effect of Tongsaimai on wound healing based on experimental and network pharmacology Author: Xu, F., Wu, J., Hu, Y., Chu, C., Liu, W., Li, X., Zheng, W., Yang, W., Zhao, B., Guo, J., Wang, Z., Jia, Y., & Xiao, W. Published: 2023 Application: WB Species: Mouse Journal: J Ethnopharmacol IF: 5.195
  • PMID:
    36983022
    dCas9-Based PDGFR-β Activation ADSCs Accelerate Wound Healing in Diabetic Mice through Angiogenesis and ECM Remodeling Author: Li, Y., Li, D., You, L., Deng, T., Pang, Q., Meng, X., & Zhu, B. Published: 2023 Application: WB Species: Mouse,Human Journal: Int J Mol Sci IF: 6.208
  • PMID:
    36291137
    Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens Author: Dong, J., Guo, C., Yang, Z., Wu, Y., & Zhang, C. Published: 2022 Application: WB Species: Hen Journal: Cells. IF: 7.666
  • PMID:
    35398393
    Estradiol reduced 5-HT reuptake by downregulating the gene expression of Plasma Membrane Monoamine Transporter(PMAT, Slc29a4) throughestrogen receptor β and the MAPK/ERK signaling pathway Author: Gu, Y., Zhang, N., Zhu, S., Lu, S., Jiang, H., & Zhou, H. Published: 2022 Application: WB Species: Rat Journal: Eur J Pharmacol IF: 4.432
  • PMID:
    35121728
    PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis Author: He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S. Published: 2022 Application: WB Species: Human Journal: Cell Death Dis IF: 8.469
  • PMID:
    35538475
    N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma Author: Chen, Y., Lu, Z., Qi, C., Yu, C., Li, Y., Huan, W., Wang, R., Luo, W., Shen, D., Ding, L., Ren, L., Xie, H., Xue, D., Wang, M., Ni, K., Xia, L., Qian, J., & Li, G. Published: 2022 Application: WB Species: Human Journal: Mol Cancer. IF: 41.444
  • PMID:
    35950373
    Downregulation of fibulin-4 inhibits autophagy and promotes the sensitivity of esophageal squamous cell carcinoma cells to apatinib by activating the Akt-mTOR signaling pathway Author: Chen, X., Wang, J., Song, L., Yu, Y., Shi, M., Jiang, W., Liu, X., & He, X. Published: 2022 Application: WB Species: Human Journal: Thorac Cancer. IF: 3.223
  • PMID:
    35794161
    PD-L1 regulates cell proliferation and apoptosis in acute myeloid leukemia by activating PI3K-AKT signaling pathway Author: Wang, F., Yang, L., Xiao, M., Zhang, Z., Shen, J., Anuchapreeda, S., Tima, S., Chiampanichayakul, S., & Xiao, Z. Published: 2022 Application: WB Journal: Sci Rep. IF: 4.380
  • PMID:
    34541418
    Aligned fibrin/functionalized self-assembling peptide interpenetrating nanofiber hydrogel presenting multi-cues promotes peripheral nerve functional recovery Author: Yang, S., Zhu, J., Lu, C., Chai, Y., Cao, Z., Lu, J., Zhang, Z., Zhao, H., Huang, Y. Y., Yao, S., Kong, X., Zhang, P., & Wang, X. Published: 2021 Application: WB Species: Rat Journal: Bioact Mater IF: 14.594
  • PMID:
    32236608
    CD44 inhibition attenuates EGFR signaling and enhances cisplatin sensitivity in human EGFR wild-type non-small-cell lung cancer cells Author: Dandan Yuan;Mingxing Li Published: 2020 Application: WB,IF Species: human Journal: Int J Mol Med. IF: 2.928
  • PMID:
    33294120
    Metformin Prevents Follicular Atresia in Aging Laying Chickens through Activation of PI3K/AKT and Calcium Signaling Pathways Author: Jian Li; Caiqiao Zhang Published: 2020 Application: WB,IF,IHC Species: hen Journal: Oxid Med Cell Longev. IF: 5.076
  • PMID:
    32929062
    Inhibition of the activation of γδT17 cells throughPPARγ–PTEN/Akt/GSK3β/NFAT pathwaycontributes to the anti-colitis effect of madecassicacid Author: Yun, X., Fang, Y., Lv, C., Qiao, S., Tao, Y., Dai, Y., & Xia, Y. Published: 2020 Application: WB Species: Mouse Journal: Cell Death and Disease IF: 6.30
  • PMID:
    31740942
    Knockdown of ISOC1 inhibits the proliferation and migration and induces the apoptosis of colon cancer cells through the AKT/GSK-3β pathway Author: ,Wang GuiYing Published: 2019 Application: WB Species: human Journal: Carcinogenesis. IF: 4.004

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