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石蜡组织切片免疫荧光(IF-tissue)

石蜡组织切片免疫荧光(IF-tissue)操作流程
1. 组织切片60℃烤片溶蜡;
2. 二甲苯(I)脱蜡15min;二甲苯(II)脱蜡15min;(抗原修复参考IHC实验流程)
3. 0.25%Triton X-100+1%FBS+10%山羊血清(TBS) 通透封闭孵育20min。
4. 一抗孵育4℃过夜或37℃ 1h。阴性对照最好用一抗来源血清,否则用PBS液。
5. TBST清洗标本 3次各3min。
6. 荧光二抗孵育37℃ 1h(室温高于22℃则室温孵育)。
7. 蒸馏水洗2次1min。
8. TBST清洗标本3次各3min。
9. 抗荧光淬灭封片剂封片。

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