ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36] - BSA and Azide free
Catalog# HA750418
ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human ULK1 aa 930-979 / 1050.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 113 kDa
阳性对照
A-172 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, MCF7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, HepG2 cell lysate, C2C12 cell lysate, MEF cell lysate, PC-12 cell lysate, human spleen tissue, rat skeletal muscle tissue, human colon carcinoma tissue, mouse brain tissue.
偶联
unconjugated
克隆号
JA58-36
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:50-1:200
靶点
功能
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.
背景文献
1. Desantis, A. et al. Che-1-induced inhibition of mTOR pathway enables stress-induced autophagy. The EMBO journal. 34: 1214-30 (2015).
2. Xiong, H. et al. Activation of miR-34a/SIRT1/p53 signaling contributes to cochlear hair cell apoptosis: implications for age-related hearing loss. Neurobiology of aging. 36: 1692-701 (2015).
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
组织特异性
Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
翻译后修饰
Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.; Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.
亚细胞定位
Cytosol, Preautophagosomal structure.
别名
ATG 1 antibody
ATG1 antibody
ATG1 autophagy related 1 homolog antibody
ATG1A antibody
Autophagy related protein 1 homolog antibody
Autophagy-related protein 1 homolog antibody
FLJ38455 antibody
FLJ46475 antibody
hATG1 antibody
KIAA0722 antibody
展开图片
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Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (HA750418) at 1/5,000 dilution.
Lane 1: A-172 cell lysate (15 µg/Lane)
Lane 2: SH-SY5Y cell lysate (15 µg/Lane)
Lane 3: U-2 OS cell lysate (15 µg/Lane)
Lane 4: MCF7 cell lysate (15 µg/Lane)
Lane 5: Mouse spleen tissue lysate (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 113 kDa
Observed band size: 130 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750418) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ULK1 on different lysates with Rabbit anti-ULK1 antibody (HA750418) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: C2C12 cell lysate
Lane 3: MEF cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 113 kDa
Observed band size: 130 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750418) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750418, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ULK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750418, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ULK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750418, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ULK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750418, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
ULK1 Recombinant Rabbit Monoclonal Antibody [JA58-36]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
