TLR4 Mouse Monoclonal Antibody
Catalog# RT1666
TLR4 Mouse Monoclonal Antibody
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WB
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Human
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Mouse
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Rat
概述
产品名称
TLR4 Mouse Monoclonal Antibody
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within mouse TLR4.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 96 kDa
阳性对照
Jurkat cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Store at +4℃
存储缓冲液
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Toll-like receptor 4 (TLR4), also designated as CD284 (cluster of differentiation 284), is a key activator of the innate immune response and plays a central role in the fight against bacterial infections. TLR4 is a transmembrane protein of approximately 95 kDa that is encoded by the TLR4 gene. TLR4 belongs to the toll-like receptor family which is representative of the pattern recognition receptors (PRR), so named for their ability to recognize evolutionarily conserved components of microorganisms (bacteria, viruses, fungi and parasites) called pathogen-associated molecular patterns (PAMPs). The recognition of a PAMP by a PRR triggers rapid activation of the innate immunity essential to fight infectious diseases.
背景文献
1. Ciesielska A et al. TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cell Mol Life Sci. 2021 Feb
2. Zhang Y et al. Toll-like receptor 4 (TLR4) inhibitors: Current research and prospective. Eur J Med Chem. 2022 May
序列相似性
Belongs to the Toll-like receptor family.
组织特异性
Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
翻译后修饰
N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.; Phosphorylated on tyrosine residues by LYN after binding lipopolysaccharide.
亚细胞定位
Cell membrane, Early endosome, Cell projection, ruffle.
别名
ARMD10 antibody
CD284 antibody
CD284 antigen antibody
Homolog of Drosophila toll antibody
hToll antibody
TLR 4 antibody
TLR4 antibody
TLR4_HUMAN antibody
TOLL antibody
Toll like receptor 4 antibody
展开图片
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Western blot analysis of TLR4 on different lysates with Mouse anti-TLR4 antibody (RT1666) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (30 µg/Lane)
Lane 2: THP-1 cell lysate (30 µg/Lane)
Lane 3: RAW264.7 cell lysate (30 µg/Lane)
Lane 4: Neuro-2a cell lysate (30 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Lane 7: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 96 kDa
Observed band size: 96 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (RT1666) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"