NF-kB p100 / NFKB2 Recombinant Rabbit Monoclonal Antibody [JM82-03]
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Catalog# ET1705-45
NF-kB p100 / NFKB2 Recombinant Rabbit Monoclonal Antibody [JM82-03]
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
概述
产品名称
NF-kB p100 / NFKB2 Recombinant Rabbit Monoclonal Antibody [JM82-03]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human NFKB2 aa 725-897 / 900.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, IP
分子量
Predicted band size: 97 kDa
阳性对照
MCF7 cell lysate, THP-1 cell lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat spleen tissue lysate, A431, human colon cancer tissue, mouse thymus tissue, human tonsil tissue, human placenta tissue.
偶联
unconjugated
克隆号
JM82-03
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
靶点
功能
Nuclear factor NF-kappa-B p100 subunit is a protein that in humans is encoded by the NFKB2 gene. NF-κB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NF-κB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth.
背景文献
1. Basu, A. et al. Vanadium(III)-L-cysteine protects cisplatin-induced nephropathy through activation of Nrf2/HO-1 pathway. Free radical research 50: 39-55 (2016).
2. Wu, SM. et al. Melatonin set out to ER stress signaling thwarts epithelial mesenchymal transition and peritoneal dissemination via Calpain-mediated C/EBPβ and NFκB cleavage. Journal of pineal research (2015).
翻译后修饰
While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.; Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.; Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.
亚细胞定位
Cytoplasm. Nucleus.
别名
CVID10 antibody
DNA binding factor KBF2 antibody
H2TF1 antibody
Lymphocyte translocation chromosome 10 protein antibody
LYT 10 antibody
NF kB2 antibody
NFKB p52/p100 subunit antibody
Nuclear factor Kappa B subunit 2 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
展开图片
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Western blot analysis of NF-kB p100 / NFKB2 on different lysates with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)
Lane 3: Mouse kidney tissue lysate (30 µg/Lane)
Lane 4: Rat heart tissue lysate (30 µg/Lane)
Lane 5: Rat spleen tissue lysate (30 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 110 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-45) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of NF-kB p100 / NFKB2 on different lysates with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NF-kB p100 / NFKB2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 110 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-45) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling NF-kB p100 / NFKB2 with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-45) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-NF-kB p100 / NFKB2 antibody (ET1705-45) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
