MMP-2 Mouse Monoclonal Antibody [B11-E6]
Catalog# EM1706-99
MMP-2 Mouse Monoclonal Antibody [B11-E6]
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WB
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FC
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Human
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Mouse
概述
产品名称
MMP-2 Mouse Monoclonal Antibody [B11-E6]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human MMP2 aa 9-140.
种属反应性
Human, Mouse
验证应用
WB, FC
分子量
72 kDa
阳性对照
Human MMP2 recombinant protein, MMP2-hIgGFc transfected HEK293, MCF-7, Raw264.7, HUVEC, T47D, Hela.
偶联
unconjugated
克隆号
B11-E6
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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FC
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1:100-1:200
发表文章中的应用
| IF | 查看 1 篇文献如下 |
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Mouse | 查看 2 篇文献如下 |
| Human | 查看 1 篇文献如下 |
靶点
功能
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
背景文献
1. Zhong J et al. TGF- 1 induces HMGA1 expression: The role of HMGA1 in thyroid cancer proliferation and invasion. Int J Oncol 50:1567-1578 (2017).
2. Xu L et al. Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat uterine scars. Stem Cell Res Ther 8:84 (2017).
亚细胞定位
Secreted. Membrane. Nucleus
别名
72 kDa gelatinase antibody
72kD type IV collagenase antibody
CLG 4 antibody
CLG 4A antibody
CLG4 antibody
CLG4A antibody
Collagenase Type 4 alpha antibody
Collagenase type IV A antibody
Gelatinase A antibody
Gelatinase alpha antibody
展开图片
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Western blot analysis of MMP2 on human MMP2 recombinant protein using anti-MMP2 antibody at 1/1,000 dilution.
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Western blot analysis of MMP2 on HEK293 (1) and MMP2-hIgGFc transfected HEK293 (2) cell lysate using anti-MMP2 antibody at 1/1,000 dilution.
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Western blot analysis of MMP2 on different cell lysate using anti-MMP2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: Raw264.7
Lane 3: HUVEC
Lane 4: T47D -
Flow cytometric analysis of Hela cells with MMP-2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway
Author: Jiang Huangyu,et al
PMID: no pmid0505
应用: WB
反应种属: Human,Mouse
发表时间: 2024 Apr
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Citation
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RCAN1.4 suppresses the osteosarcoma growth and metastasis via interfering with the calcineurin/NFAT signaling pathway. Journal of bone oncology, 30, 100383.
Author: Huang, B., Jiang, Z., Wu, S., Wu, H., Zhang, X., Chen, J., Zhao, F., & Liu, J.
PMID: 34336566
应用: IF
反应种属: Mouse
发表时间: 2021 Jul
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Citation
Alternative Products
MMP-2 Recombinant Rabbit Monoclonal Antibody [SI15-04]
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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