CHD1 Mouse Monoclonal Antibody [2-2]
Catalog# M1211-5
CHD1 Mouse Monoclonal Antibody [2-2]
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WB
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IHC-P
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Human
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Mouse
概述
产品名称
CHD1 Mouse Monoclonal Antibody [2-2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human CHD1 aa 1,479-1,710 / 1,710.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
Predicted band size: 197 kDa
阳性对照
HeLa cell lysate, HEK-293 cell lysate, MOLT-4 cell lysate, COS-1 cell lysate, mouse testis tissue.
偶联
unconjugated
克隆号
2-2
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:100
靶点
功能
Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms.This family of proteins, which consists of nine members, has been divided into three separate subfamilies.All of the CHD proteins contain two tandem N-terminal chromodomains, a SWI/SNF-related ATPase domain, and a C-terminal DNA binding domain.CHD1 may play an important role in gene regulation.
背景文献
1. Double chromodomains cooperate to recognize the methylated histone H3 tail."Flanagan J.F., Mi L.-Z., Chruszcz M., Cymborowski M., Clines K.L., Kim Y., Minor W., Rastinejad F., Khorasanizadeh S.Nature 438:1181-1185(2005)
2. Human but not yeast CHD1 binds directly and selectively to histone H3 methylated at lysine 4 via its tandem chromodomains."Sims R.J. III, Chen C.-F., Santos-Rosa H., Kouzarides T., J. Biol. Chem. 280:41789-41792(2005)
序列相似性
Belongs to the SNF2/RAD54 helicase family.
组织特异性
Expressed in many tissues including in the brain, where the highest level of expression is found in the cerebellum and basal ganglia.
亚细胞定位
Nucleus, Cytoplasm.
别名
ATP dependent helicase CHD 1 antibody
ATP-dependent helicase CHD1 antibody
CHD 1 antibody
CHD-1 antibody
Chd1 antibody
CHD1_HUMAN antibody
Chromodomain helicase DNA binding protein 1 antibody
Chromodomain-helicase-DNA-binding protein 1 antibody
DKFZp686E2337 antibody
OTTHUMP00000222625 antibody
图片
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Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MOLT-4 cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 197 kDa
Observed band size: 260 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CHD1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 197 kDa
Observed band size: 260 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis on recombinant protein using anti-CHD1 Mouse mAb (Cat. # M1211-5).
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Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CHD1 Mouse mAb (Cat. # M1211-5).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"