Lentivirus HIV-1 p24 enzyme-linked immunoassay kit
Catalog# EHY0002
Lentivirus HIV-1 p24 enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
Standard curve range
-
4.12~3000 ng/ml
概述
产品名称
Lentivirus HIV-1 p24 enzyme-linked immunoassay kit
产品类型
Elisa Kit
灵敏度
0.12 ng/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0072 | 0.0068 | 0.0070 | |
| 4.12 | 0.0220 | 0.0212 | 0.0216 | 0.0146 |
| 12.35 | 0.0453 | 0.0461 | 0.0457 | 0.0387 |
| 37.04 | 0.1085 | 0.1066 | 0.1076 | 0.1006 |
| 111.11 | 0.2794 | 0.2775 | 0.2785 | 0.2715 |
| 333.33 | 0.6978 | 0.6874 | 0.6926 | 0.6856 |
| 1000.00 | 1.7410 | 1.6832 | 1.7121 | 1.7051 |
| 3000.00 | 2.7224 | 2.6821 | 2.7023 | 2.6953 |
精度
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 62.5 | 333.8 | 928.4 | 62.6 | 324.3 | 978.5 |
| Standard Deviation | 3.1 | 15.9 | 58.3 | 1.8 | 6.0 | 39.4 |
| Coefficient of Variation (%) | 4.9 | 4.8 | 6.3 | 3.9 | 1.9 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
加标回收
通过加标 3 个水平的 HIV-1 p24 来评估加标回收。回收率从95%到106%不等，总体平均回收率为101%。
样本值
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum |
实验类型
Sandwich Elisa
种属反应性
检测方法
Colorimetric
操作时长
75min
标准曲线梯度
7点/3倍
孵育次数
1
加样体积
10 μl
产品特性
储存条件
存储于 2-8 ℃
靶点
功能
人类免疫缺陷病毒 1 （HIV-1） 的 gag 基因编码一种称为 Pr55Gag 的前体蛋白。病毒蛋白酶 PR 切割该前体以产生病毒颗粒组装所需的 p17、p24、p7 和 p6 蛋白。p24 是一种主要的病毒核心结构蛋白。其测量通常用作 HIV-1 感染和病毒载量的指标。