Biotin Conjugated Human IL3RA/CD123 Recombinant Rabbit Monoclonal Antibody [PSH11-58] - Detector
概述
产品名称
Biotin Conjugated Human IL3RA/CD123 Recombinant Rabbit Monoclonal Antibody [PSH11-58] - Detector
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human IL3RA/CD123 aa 19-305 (HA211048).
种属反应性
Human
验证应用
ELISA(Det), ELISA
阳性对照
Recombinant Human IL3RA/CD123 protein (HA211048).
偶联
Biotin
克隆号
PSH11-58
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH11-57] to Human IL3RA/CD123 antibody (Capture) (HA723351) and Recombinant Human IL3RA/CD123 protein (HA211048) as the standard. The reference range value is 0.625-20 ng/mL.
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ELISA
-
Use at an assay dependent concentration.
靶点
功能
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.
背景文献
1. Huang S., Chen Z., Yu J.F., Young D., Bashey A., Ho A.D., Law P. Correlation between IL-3 receptor expression and growth potential of human CD34+ hematopoietic cells from different tissues. Stem Cells 17:265-272 (1999)
2. Broughton S.E., Hercus T.R., Nero T.L., Kan W.L., Barry E.F., Dottore M., Cheung Tung Shing K.S., Morton C.J., Dhagat U., Parker M.W. A dual role for the N-terminal domain of the IL-3 receptor in cell signalling. Nat. Commun. 9:386-386 (2018)
亚细胞定位
Membrane.
UNIPROT
别名
CD123 antibody
CD123 antigen antibody
hIL 3Ra antibody
hIL-3Ra antibody
hIL3Ra antibody
IL 3 receptor alpha SP2 isoform antibody
IL 3R alpha antibody
IL-3 receptor subunit alpha antibody
IL-3R subunit alpha antibody
IL-3R-alpha antibody
展开图片
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Sandwich ELISA analysis of human CD123 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723351) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL3RA/CD123 protein (HA211048) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723353B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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