ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00]
Catalog# HA723267
ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00]
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IHC-P
-
WB
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Mouse
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Rat
概述
产品名称
ROR alpha / RORA Recombinant Rabbit Monoclonal Antibody [PSH11-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse RORA aa aa 101-523.
种属反应性
Mouse, Rat
验证应用
IHC-P, WB
分子量
Predicted band size: 59 kDa
阳性对照
Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH11-00
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
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1:1,000
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WB
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1:2,000
靶点
功能
RAR-related orphan receptor alpha (RORα), also known as NR1F1 (nuclear receptor subfamily 1, group F, member 1) is a nuclear receptor that in humans is encoded by the RORA gene. RORα participates in the transcriptional regulation of some genes involved in circadian rhythm. In mice, RORα is essential for development of cerebellum through direct regulation of genes expressed in Purkinje cells. It also plays an essential role in the development of type 2 innate lymphoid cells (ILC2) and mutant animals are ILC2 deficient. In addition, although present in normal numbers, the ILC3 and Th17 cells from RORα deficient mice are defective for cytokine production.
背景文献
1. Liu D et al. The Circadian Clock Component RORA Increases Immunosurveillance in Melanoma by Inhibiting PD-L1 Expression. Cancer Res. 2024 Jul
2. Li M et al. The single-cell transcriptomic atlas and RORA-mediated 3D epigenomic remodeling in driving corneal epithelial differentiation. Nat Commun. 2024 Jan
亚细胞定位
Nucleus.
别名
fhl2a antibody
NR1F1 antibody
Nuclear receptor ROR alpha antibody
Nuclear receptor ROR-alpha antibody
Nuclear receptor RZR-alpha antibody
Nuclear receptor subfamily 1 group F member 1 antibody
RAR related orphan receptor A antibody
RAR related orphan receptor alpha antibody
RAR-related orphan receptor A antibody
Retinoid-related orphan receptor-alpha antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-ROR alpha / RORA antibody (HA723267) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723267) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-ROR alpha / RORA antibody (HA723267) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723267) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of ROR alpha / RORA on different lysates with Rabbit anti-ROR alpha / RORA antibody (HA723267) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 3: Mouse spleen tissue lysate (negative) (40 µg/Lane)
Lane 4: Rat cerebellum tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 56/59 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723267) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"