Human Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH06-45] - BSA and Azide free (Capture)
概述
产品名称
Human Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH06-45] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Complement C5 19-821.
种属反应性
Human
验证应用
ELISA(Cap)
阳性对照
Recombinant standard Human Complement C5 protein (HA210636).
偶联
unconjugated
克隆号
PSH06-45
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-46] to Human Complement C5 antibody (Detector) (HA722683) and recombinant standard Human Complement C5 protein (HA210636). The reference range value is 111-9,000pg/ml.
靶点
功能
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
背景文献
1. DeMartino J.A., Van Riper G., Siciliano S.J., Molineaux C.J., Konteatis Z.D., Rosen H., Springer M.S. The amino terminus of the human C5a receptor is required for high affinity C5a binding and for receptor activation by C5a but not C5a analogs. J. Biol. Chem. 269:14446-14450 (1994)
2. Hillebrandt S., Wasmuth H.E., Weiskirchen R., Hellerbrand C., Keppeler H., Werth A., Schirin-Sokhan R., Wilkens G., Geier A., Lammert F. Complement factor 5 is a quantitative trait gene that modifies liver fibrogenesis in mice and humans. Nat. Genet. 37:835-843 (2005)
亚细胞定位
Secreted.
UNIPROT
别名
Anaphylatoxin C5a analog antibody
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
C5 antibody
C5a anaphylatoxin antibody
C5a antibody
C5b antibody
CO5_HUMAN antibody
Complement C5 alpha'' chain antibody
Complement C5 antibody
Complement component C5 antibody
展开图片
-
Sandwich ELISA analysis of human Complement C5 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722682) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human Complement C5 protein starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722683, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentration of native Complement C5 was measured in duplicate at different sample concentrations. Undiluted samples were 1:10000 serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Complement C5 concentration was determined to be 78.3 ug/mL in human serum.
-
Interpolated concentrations of native Complement C5 in human cell culture supernatant samples.
Complement C5 was measured in 100% cell supernatant. The concentrations of Complement C5 were interpolated from the Complement C5 standard curves. The mean Complement C5 concentration was determined to be 5.256 ng/mL in HepG2 cell supernanant. There was no detectable signal in U-2 OS cell supernatant. -
Interpolated concentrations of spiked Complement C5 in human cell culture media samples.
The concentrations of Complement C5 were measured in duplicates, interpolated from the Complement C5 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"