Phospho-PLC-gamma-1 (Y783) Recombinant Rabbit Monoclonal Antibody [PSH06-29]
Catalog# HA722661
Phospho-PLC-gamma-1 (Y783) Recombinant Rabbit Monoclonal Antibody [PSH06-29]
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WB
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Human
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Mouse
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Rat
概述
产品名称
Phospho-PLC-gamma-1 (Y783) Recombinant Rabbit Monoclonal Antibody [PSH06-29]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLC-gamma-1.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 149 kDa
阳性对照
A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate.
偶联
unconjugated
克隆号
PSH06-29
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
Phospholipase C, gamma 1, also known as PLCG1 and PLCgamma1, is a protein that in humans involved in cell growth, migration, apoptosis, and proliferation. It is encoded by the PLCG1 gene and is part of the PLC superfamily. PLCγ1 is a cell growth factor from the PLC superfamily. PLCγ1 is used during the cell growth and in a cell migration and apoptosis, all of which are vital cell processes that, if disrupted by mutations, can cause cancerous cells to form within the body. Mutations in this protein show an increase in issues in cells regarding regulation of proliferation and their cell signaling. PLCγ1 roles are also involved in neuronal actin growth, calcium signaling, and brain development. It is highly regulated by multiple factors, such as PIK3, AMPK, and FAK. It is part of the PIP3 pathway and leads to and increase in calcium in the cells. In neuronal cells, PLCγ1 is highly involved in actin cytoskeleton organization and synaptic plasticity.
背景文献
1. Tao P et al. A gain-of-function variation in PLCG1 causes a new immune dysregulation disease. J Allergy Clin Immunol. 2023 Nov
2. Schnoeder TM et al. PLCG1 is required for AML1-ETO leukemia stem cell self-renewal. Blood. 2022 Feb
亚细胞定位
Cell projection, lamellipodium, ruffle.
别名
1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
Inositoltrisphosphohydrolase antibody
Monophosphatidylinositol phosphodiesterase antibody
NCKAP3 antibody
Phosphatidylinositol phospholipase C antibody
Phosphoinositidase C antibody
Phosphoinositide phospholipase C antibody
Phosphoinositide phospholipase C-gamma-1 antibody
展开图片
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☑ Cell treatment (CT)
Western blot analysis of Phospho-PLC-gamma-1 (Y783) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (Y783) antibody (HA722661) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate
Lane 5: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 149 kDa
Observed band size: 149 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722661) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"