Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52]
Catalog# HA722294
Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52]
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WB
-
IHC-P
-
Human
-
Mouse
-
Rat
概述
产品名称
Niemann Pick C2 Recombinant Rabbit Monoclonal Antibody [PSH05-52]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Niemann Pick C2 aa 1-151 / 151.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 17 kDa
阳性对照
HepG2 cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
偶联
unconjugated
克隆号
PSH05-52
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-P
-
1:100,000-1:300,000
靶点
功能
The epididymal secretory protein E1, also known as NPC2 (Niemann-Pick intracellular cholesterol transporter 2), is one of two main lysosomal transport proteins that assist in the regulation of cellular cholesterol by exportation of LDL-derived cholesterol from lysosomes. Lysosomes have digestive enzymes that allow it to break down LDL particles to LDL-derived cholesterol once the LDL particle is engulfed into the cell via receptor mediated endocytosis. NPC2 works cooperatively with the NPC1 protein to facilitate the exportation of LDL-derived cholesterol out of the lysosome to regulate the concentrations of lipids and cholesterol in the body. Epididymal secretory protein E1 is a protein associated with Niemann-Pick disease, type C, which is one of the 3 types of the Niemann-Pick diseases (Type A, B, and C). This disease can lead to an over accumulation of cholesterol and lipids in different types of tissues, including the brain. It is caused by a mutation in the NPC2 gene that impairs the body's ability to transport lipids or cholesterol intracellularly.
背景文献
1. Tseng WC et al. The role of Niemann-Pick type C2 in zebrafish embryonic development. Development. 2021 Apr
2. Juhl AD et al. Niemann Pick C2 protein enables cholesterol transfer from endo-lysosomes to the plasma membrane for efflux by shedding of extracellular vesicles. Chem Phys Lipids. 2021 Mar
亚细胞定位
Secreted, Endoplasmic reticulum, Lysosome.
别名
EDDM1 antibody
Epididymal protein 1 antibody
Epididymal secretory protein antibody
Epididymal secretory protein E1 antibody
hE1 antibody
Human epididymis-specific protein 1 antibody
Niemann-Pick disease type C2 antibody
Niemann-Pick disease type C2 protein antibody
NPC intracellular cholesterol transporter 2 antibody
NPC2 antibody
展开图片
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Western blot analysis of Niemann Pick C2 on different lysates with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HEK-293 cell lysate (10 µg/Lane)
Lane 3: RAW264.7 cell lysate (10 µg/Lane)
Lane 4: C2C12 cell lysate (10 µg/Lane)
Lane 5: C6 cell lysate (10 µg/Lane)
Lane 6: Mouse kidney tissue lysate (20 µg/Lane)
Lane 7: Mouse spleen tissue lysate (20 µg/Lane)
Lane 8: Rat kidney tissue lysate (20 µg/Lane)
Lane 9: Rat spleen tissue lysate (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 16-18 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722294) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/100,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/100,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/100,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Niemann Pick C2 antibody (HA722294) at 1/300,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722294) at 1/300,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"