Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH04-67] - BSA and Azide free (Detector)
概述
产品名称
Mouse IL-1 beta Recombinant Rabbit Monoclonal Antibody [PSH04-67] - BSA and Azide free (Detector)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Mouse IL-1 beta aa 118-269 (P10749).
种属反应性
Mouse
验证应用
ELISA(Det)
分子量
Predicted band size: 31 kDa
阳性对照
Recombinant Mouse IL-1β protein (HA210706).
偶联
unconjugated
克隆号
PSH04-67
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-66] to Mouse IL-1 beta (Capture) (HA722166) and recombinant standard Mouse IL-1 beta (HA210706). The reference range value is 12.3-3000 pg/ml.
靶点
功能
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages, monocytes, and a subset of dentritic cells known as slanDC, as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
背景文献
1. Bertoni A et al. Spontaneous NLRP3 inflammasome-driven IL-1-β secretion is induced in severe COVID-19 patients and responds to anakinra treatment. J Allergy Clin Immunol. 2022 Oct
2. Zhang J et al. NLRP3 inflammasome mediates M1 macrophage polarization and IL-1β production in inflammatory root resorption. J Clin Periodontol. 2020 Apr
亚细胞定位
Cytoplasm, cytosol, Secreted, Lysosome, extracellular exosome.
UNIPROT
别名
Catabolin antibody
H1 antibody
IFN beta inducing factor antibody
IL 1 antibody
IL 1 beta antibody
IL-1 beta antibody
IL1 antibody
IL1 BETA antibody
IL1B antibody
IL1B_HUMAN antibody
展开图片
-
Sandwich ELISA analysis of Mouse IL-1β matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722166) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-1β protein (HA210706) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722167, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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