p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60]
Catalog# HA722019
p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60]
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WB
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Human
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Mouse
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Rat
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Monkey
概述
产品名称
p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody [PSH03-60]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human MAPK12 aa 1-367 / 367.
产品特异性
Anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Antibody [PSH03-60] detects endogenous levels of total p38α, p38β and p38γ MAPK protein. This antibody does not recognize p38δ MAPK protein.
种属反应性
Human, Mouse, Rat, Monkey
验证应用
WB
分子量
Predicted band size: 42 kDa
阳性对照
293T cell lysate, HeLa cell lysate, K-562 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue lysate.
偶联
unconjugated
克隆号
PSH03-60
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
靶点
功能
A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs).
背景文献
1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct
2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun
亚细胞定位
Cytoplasm, Nucleus, Mitochondrion.
UNIPROT
别名
ERK 6 antibody
ERK-6 antibody
ERK6 antibody
Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody
MAPK 12 antibody
Mapk12 antibody
Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase p38 gamma antibody
展开图片
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Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution.
Lane 1: 293T cell lysate (30 µg/Lane)
Lane 2: HeLa cell lysate (30 µg/Lane)
Lane 3: K-562 cell lysate (30 µg/Lane)
Lane 4: MDA-MB-231 cell lysate (30 µg/Lane)
Lane 5: COS-1 cell lysate (30 µg/Lane)
Lane 6: Neuro-2a cell lysate (30 µg/Lane)
Lane 7: PC-12 cell lysate (30 µg/Lane)
Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane)
Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane)
Lane 10: Rat heart tissue lysate (30 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody (HA722019) at 1/2,000 dilution.
Lane 1: Human p38 gamma recombinant protein, 30ng/Lane
Lane 2: Human p38 alpha recombinant protein, 50ng/Lane
Lane 3: Human p38 delta recombinant protein, 50ng/Lane
Lane 4: Human p38 beta recombinant protein, 50ng/Lane
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722019) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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