iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
Catalog# HA720141F
iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
-
IF-Tissue
-
Human
-
Mouse
-
Rat
概述
产品名称
iFluor™ 647 Conjugated Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody [SN72-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within mouse Cytokeratin 1 aa 586-637.
种属反应性
Human, Mouse, Rat
验证应用
IF-Tissue
分子量
Predicted band size: 66 kDa
阳性对照
Human skin tissue.
偶联
iFluor™ 647
克隆号
SN72-08
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IF-Tissue
-
1:100-1:400
靶点
功能
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis.
背景文献
1. Zhu, H. et al. 2016. MiR-217 is down-regulated in psoriasis and promotes keratinocyte differentiation via targeting GRHL2. Biochemical and biophysical research communications. 471: 169-76.
2. Li, G. et al. 2015. Transcription Factor PAX6 (Paired Box 6) Controls Limbal Stem Cell Lineage in Development and Disease. The Journal of biological chemistry. 290: 20448-54.
亚细胞定位
Cell membrane.
别名
67 kDa cytokeratin antibody
CK-1 antibody
CK1 antibody
Cytokeratin-1 antibody
Cytokeratin1 antibody
EHK antibody
EHK1 antibody
Epidermolytic hyperkeratosis 1 antibody
EPPK antibody
Hair alpha protein antibody
展开图片
-
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F), Cytokeratin 5 (HA720129F) and Cytokeratin 10 (HA720119F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/400 dilution, Cytokeratin 5 (HA720129F, green) at 1/100 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS.
DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"