AP2A2 Rabbit Polyclonal Antibody
Catalog# HA500477
AP2A2 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
AP2A2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human AP2A2 aa 740-939 / 939.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
104 kDa
阳性对照
Hela cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, human kidney tissue, mouse cerebellum tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:5,000
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IHC-P
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1:200-1:1,000
靶点
功能
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10.
背景文献
1. Nelson PT. et. al. The MUC6/AP2A2 Locus and Its Relevance to Alzheimer\'s Disease: A Review. J Neuropathol Exp Neurol. 2020 Jun
2. Montgomery MK. et. al. The role of Ap2a2 in PPARalpha-mediated regulation of lipolysis in adipose tissue. FASEB J. 2019 Dec
亚细胞定位
Cell membrane, coated pit.
别名
100 kDa coated vesicle protein C antibody
Adaptor protein complex AP-2 subunit alpha-2 antibody
Adaptor-related protein complex 2 subunit alpha-2 antibody
Alpha-adaptin C antibody
Alpha2-adaptin antibody
AP-2 complex subunit alpha-2 antibody
Ap2a2 antibody
AP2A2_HUMAN antibody
Clathrin assembly protein complex 2 alpha-C large chain antibody
HIP-9 antibody
展开图片
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Western blot analysis of AP2A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500477, 1/2,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Rat brain tissue lysate -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500477, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-AP2A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500477, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"