ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
Rmb: 880 特惠 1500
Catalog# ET7106-64
ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
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WB
-
IHC-P
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IF-Tissue
-
IHC-Fr
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Human
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Mouse
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Rat
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HA750457
不含抗保成分
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Cynomolgus monkey
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Pig
预测的种属支持售后
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unconjugated
概述
产品名称
ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human ALDH1L1 aa 155-375 .
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IHC-P, IF-Tissue, IHC-Fr
靶点分子量
Predicted band size: 99 kDa
阳性对照
Human brain tissue, mouse brain tissue, rat hippocampus tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, human liver tissue, mouse liver tissue, rat liver tissue, Mouse brain tissue lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate.
偶联
unconjugated
克隆号
JU53-54
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000-1:10,000
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IHC-P
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1:500-1:2,000
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IF-Tissue
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1:500
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IHC-Fr
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1:500-1:1,000
靶点
功能
10-formyltetrahydrofolate dehydrogenase is an enzyme that in humans is encoded by the ALDH1L1 gene. The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.
背景文献
1. Tsybovsky Y et al. Structure of putative tumor suppressor ALDH1L1. Commun Biol. 2022 Jan
2. Krupenko NI et al. Knockout of Putative Tumor Suppressor Aldh1l1 in Mice Reprograms Metabolism to Accelerate Growth of Tumors in a Diethylnitrosamine (DEN) Model of Liver Carcinogenesis. Cancers (Basel). 2021 Jun
序列相似性
In the N-terminal section; belongs to the GART family.; In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
组织特异性
Highly expressed in liver, pancreas and kidney.
亚细胞定位
Cytoplasm.
别名
10 formyltetrahydrofolate dehydrogenase antibody
10 FTHFDH antibody
10-formyltetrahydrofolate dehydrogenase antibody
10-FTHFDH antibody
AL1L1_HUMAN antibody
Aldehyde dehydrogenase 1 family member L1 antibody
Aldehyde dehydrogenase family 1 member L1 antibody
aldh1l1 antibody
Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody
DKFZp781N0997 antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Application: IF-tissue
Species: Rat
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of ALDH1L1 on different lysates with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Mouse kidney tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat kidney tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-64) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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