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PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04]

RMB: 900 特惠 1500

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Catalog# ET1705-90

PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04]

Application

  • WB

  • FC

  • IP

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

预测的种属支持售后
This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human PSD93 aa 600-660.

种属反应性

Human (Predicted: Mouse, Rat)

预测的种属支持售后

验证应用

WB, FC, IP

分子量

Predicted band size: 98 kDa

阳性对照

Hela cell lysate, SH-SY5Y cell lysate, SH-SY5Y.

偶联

unconjugated

克隆号

JM41-04

RRID

AB_3070619

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

发表文章中的应用

WB 查看 2 篇文献如下

发表文章中的种属

Mouse 查看 2 篇文献如下

靶点

功能

Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.

背景文献

1. Winkler D. et. al. Hypersocial behavior and biological redundancy in mice with reduced expression of PSD95 or PSD93. Behav Brain Res. 2018 Oct

2. Shao YW. et. al. Cross-species genomics identifies DLG2 as a tumor suppressor in osteosarcoma. Oncogene. 2019 Jan

序列相似性

Belongs to the MAGUK family.

翻译后修饰

Palmitoylation of isoform 1 is not required for targeting to postsynaptic density.

亚细胞定位

Cell junction, Cell membrane, Cell projection, Membrane, Synapse.

UNIPROT

SwissProt: Q15700 Human

SwissProt: Q91XM9 Mouse

SwissProt: Q63622 Rat

别名

Channel associated protein of synapse 110 antibody

Channel associated protein of synapses 110kD antibody

Channel-associated protein of synapse-110 antibody

Chapsyn 110 antibody

Chapsyn-110 antibody

Chapsyn110 antibody

Discs, large homolog 2 (Drosophila) antibody

Disks large homolog 2 antibody

DKFZp781D1854 antibody

DKFZp781E0954 antibody

展开

图片

  • Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-90" style="font-weight: bold;text-decoration: underline;">ET1705-90</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Hela cell lysate<br />Lane 2: SH-SY5Y cell lysate

    Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: SH-SY5Y cell lysate

  • Flow cytometric analysis of PSD93 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1705-90" style="font-weight: bold;text-decoration: underline;">ET1705-90</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PSD93 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
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