Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]
Catalog# ET1701-28
Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]
-
WB
-
FC
-
Mouse
-
Rat
-
Human
预测的种属支持售后
概述
产品名称
Phospho-GluR1 (S845) Recombinant Rabbit Monoclonal Antibody [JJ2009]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser845 of Human GluR1 aa 831-880 / 906.
种属反应性
Mouse, Rat (Predicted: Human)
预测的种属支持售后
验证应用
WB, FC
分子量
Predicted band size: 102 kDa
阳性对照
Mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate the membrane treated with λpp for 1 hour, rat brain tissue lysate the membrane treated with λpp for 1 hour, N2A.
偶联
unconjugated
克隆号
JJ2009
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
FC
-
1:10-1:50
靶点
功能
Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions. The NMDA receptors consist of five subunits: epsilion 1, 2, 3, 4 and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution.
背景文献
1. He XY et al. GluA1 signal peptide determines the spatial assembly of heteromeric AMPA receptors. Proc Natl Acad Sci U S A 113:E5645-54 (2016).
2. Han M & Kim J Effect of dietary iron loading on recognition memory in growing rats. PLoS One 10:e0120609 (2015).
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
组织特异性
Widely expressed in brain.
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).; Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity). Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).
亚细胞定位
Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.
别名
GLUR 1 antibody
GLUR A antibody
AMPA 1 antibody
AMPA selective glutamate receptor 1 antibody
AMPA-selective glutamate receptor 1 antibody
GluA1 antibody
GLUH1 antibody
GluR K1 antibody
GluR-1 antibody
GluR-A antibody
展开图片
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-GluR1 (S845) on different lysates with Rabbit anti-Phospho-GluR1 (S845) antibody (ET1701-28) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour
Lane 4: Rat brain tissue lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 40 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of Phospho-GluR1 (S845) was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-28, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"