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AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

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Catalog# ET1701-26

AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

概述

产品名称

AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human EIF2C3/ Argonaute 3 aa 689-860/860.

种属反应性

Human, Mouse, Rat

验证应用

WB, IF-Cell, IF-Tissue, IHC-P, FC

分子量

Predicted band size: 97 kDa

阳性对照

HeLa cell lysate, C6 cell lysate, N2A, SHG-44, F9, NCCIT, rat spinal cord tissue, rat brain tissue, mouse brain tissue, rat testis tissue.

偶联

unconjugated

克隆号

JJ201-07

RRID

AB_3070194

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

靶点

功能

Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

背景文献

1. Hein M.Y., et al. 2015. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 163:712-723.

2. Schurmann N., et al. 2013. Molecular dissection of human Argonaute proteins by DNA shuffling. Nat. Struct. Mol. Biol. 20:818-826.

序列相似性

Belongs to the argonaute family. Ago subfamily.

亚细胞定位

Cytoplasm,Nucleus.

UNIPROT

SwissProt: Q9H9G7 Human

SwissProt: Q8CJF9 Mouse

Unigene: 92025 Rat

别名

5730550L01Rik antibody

Ago 3 antibody

Ago3 antibody

AGO3_HUMAN antibody

argonaute 3 antibody

Argonaute3 antibody

eIF 2C 3 antibody

eIF-2C 3 antibody

eIF2C 3 antibody

Eif2c3 antibody

展开

图片

  • Western blot analysis of AGO3 on different lysates with Rabbit anti-AGO3 antibody (<a href="/products/ET1701-26" style="font-weight: bold;text-decoration: underline;">ET1701-26</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-26" style="font-weight: bold;text-decoration: underline;">ET1701-26</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AGO3 on different lysates with Rabbit anti-AGO3 antibody (ET1701-26) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining AGO3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining AGO3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining AGO3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining AGO3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Flow cytometric analysis of N2A cells with AGO3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

    Flow cytometric analysis of N2A cells with AGO3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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