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Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01]

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Catalog# ET1602-17

Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Cdk5 aa 243-292 / 292.

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P

分子量

Predicted band size: 33 kDa

阳性对照

HCT 116 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human thyroid carcinoma tissue.

偶联

unconjugated

克隆号

SR39-01

RRID

AB_3069629

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:400

发表文章中的种属

Human 查看 2 篇文献如下
Mouse 查看 1 篇文献如下

靶点

功能

Cyclin dependent kinase 5 is a protein, and more specifically an enzyme, that is encoded by the Cdk5 gene. It was discovered 15 years ago and it is saliently expressed in post-mitotic central nervous system neurons (CNS). The molecule belongs to the cyclin-dependent kinase family. Kinases are enzymes that catalyze reactions of phosphorylation, a process that allows the substrate to gain a phosphate group donated by an organic compound known as ATP. Phosphorylations are of vital importance during glycolysis, therefore, making kinases an essential part of the cell due to their role in the metabolism, cell signaling and many other processes.

背景文献

1. Sadleir KR & Vassar R Cdk5 Protein Inhibition and A 42 Increase BACE1 Protein Level in Primary Neurons by a Post-transcriptional Mechanism: IMPLICATIONS OF CDK5 AS A THERAPEUTIC TARGET FOR ALZHEIMER DISEASE. J Biol Chem 287:7224-35 (2012).

2. Zhang HH et al. Increased synaptophysin is involved in inflammation-induced heat hyperalgesia mediated by cyclin-dependent kinase 5 in rats. PLoS One 7:e46666 (2012).

序列相似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

组织特异性

Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary.

翻译后修饰

Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity.; Phosphorylation at Ser-159 is essential for maximal catalytic activity.

亚细胞定位

Cytoplasm, Nucleus, Cell membrane, Perikaryon, Cell junction

UNIPROT

SwissProt: Q00535 Human

SwissProt: P49615 Mouse

SwissProt: Q03114 Rat

别名

Cdk 5 antibody

Cdk5 antibody

CDK5_HUMAN antibody

Cell division protein kinase 5 antibody

Crk6 antibody

Cyclin dependent kinase 5 antibody

Cyclin-dependent kinase 5 antibody

Protein kinase CDK5 splicing antibody

PSSALRE antibody

Serine threonine protein kinase PSSALRE antibody

展开

图片

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (<a href="/products/ET1602-17" style="font-weight: bold;text-decoration: underline;">ET1602-17</a>) at 1/500 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si Cdk5 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1602-17" style="font-weight: bold;text-decoration: underline;">ET1602-17</a>) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/500 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si Cdk5 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 30 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-17) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (<a href="/products/ET1602-17" style="font-weight: bold;text-decoration: underline;">ET1602-17</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (20 µg/Lane)<br />Lane 2: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 3: PC-12 cell lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (30 µg/Lane)<br />Lane 5: Rat brain tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 33 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1602-17" style="font-weight: bold;text-decoration: underline;">ET1602-17</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cdk5 on different lysates with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate (20 µg/Lane)
    Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 3: PC-12 cell lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (30 µg/Lane)
    Lane 5: Rat brain tissue lysate (30 µg/Lane)

    Predicted band size: 33 kDa
    Observed band size: 30 kDa

    Exposure time: 42 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Cdk5 antibody (<a href="/products/ET1602-17" style="font-weight: bold;text-decoration: underline;">ET1602-17</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-17" style="font-weight: bold;text-decoration: underline;">ET1602-17</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Cdk5 antibody (ET1602-17) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-17) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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