MST1 Rabbit Polyclonal Antibody
Catalog# ER1913-22
MST1 Rabbit Polyclonal Antibody
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IHC-P
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IF-Cell
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IF-Tissue
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FC
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Human
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Rat
概述
产品名称
MST1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human MST1 Cterminus 385-487/487
种属反应性
Human, Rat
验证应用
IHC-P, IF-Cell, IF-Tissue, FC
分子量
56 KDa
阳性对照
Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:100-500
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IF-cell
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1:25
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IF-tissue
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1:100-500
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FC
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2ug/Test
靶点
功能
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells, and to inhibit tumor formation. Phosphorylates 'Ser-14' of histone H2B during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 and alters its binding affinity to TNNC1 and TNNT2. Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner.
背景文献
暂无
亚细胞定位
Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm.
UNIPROT
别名
Kinase responsive to stress antibody
Krs2 antibody
Mammalian STE20 like protein kinase 1 antibody
Mammalian STE20-like protein kinase 1 antibody
Mammalian sterile 20 like 1 antibody
MST-1 antibody
MST1 antibody
Serine/threonine kinase 4 antibody
Serine/threonine protein kinase Krs 2 antibody
Serine/threonine-protein kinase 4 antibody
展开图片
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Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MST1) Polyclonal Antibody, Unconjugated (ER1913-22) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MST1 Polyclonal Antibody, Unconjugated(ER1913-22) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Tissue/cell: Human laryngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MST1 Polyclonal Antibody, Unconjugated(ER1913-22) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
Tissue/cell: Human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MST1 Polyclonal Antibody, Unconjugated(ER1913-22) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining -
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MST1) polyclonal Antibody, Unconjugated (ER1913-22) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
STK4 / MST-1 Rabbit Polyclonal Antibody
Application: WB,IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
