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Myelin Basic Protein Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# ER1912-82

Myelin Basic Protein Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Pig

  • Guinea Pig

概述

产品名称

Myelin Basic Protein Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

KLH conjugated synthetic peptide derived from Gpig MBP: 69-85/167

种属反应性

Human, Mouse, Rat, Pig, Guinea Pig

验证应用

WB, IHC-P, IF-Tissue, FC

分子量

33 kDa

阳性对照

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

偶联

unconjugated

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

存储缓冲液

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:400-1:800

  • IF-Tissue

  • 1:100-1:500

  • FC

  • 1ug/test

靶点

功能

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

背景文献

暂无

亚细胞定位

Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.

UNIPROT #

P02686

别名

GDB antibody

Golli MBP antibody

Golli MBP

myelin basic protein antibody

Hemopoietic MBP antibody

HMBPR antibody

HUGO antibody

MBP antibody

MBP_CAVPO antibody

MBP_HUMAN antibody

展开

图片

  • 25 ug total protein per lane of various lysates (see on figure) probed with MBP polyclonal antibody, unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:1,000 dilution and 4℃ overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

    25 ug total protein per lane of various lysates (see on figure) probed with MBP polyclonal antibody, unconjugated (ER1912-82) at 1:1,000 dilution and 4℃ overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red, bs-0295D-Cy5), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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