Thyroid Peroxidase(TPO) Mouse Monoclonal Antibody
Catalog# EM60135
Thyroid Peroxidase(TPO) Mouse Monoclonal Antibody
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IHC-P
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Human
概述
产品名称
Thyroid Peroxidase(TPO) Mouse Monoclonal Antibody
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthesized peptide derived from human Thyroid Peroxidase(TPO)
种属反应性
Human
验证应用
IHC-P
分子量
103 kDa
阳性对照
Human follicular thyroid carcinoma tissue, human papillary thyroid carcinoma tissue.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化方式
Immunogen affinity purified.
应用稀释度
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IHC-P
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1:100-1:500
靶点
功能
Thyroid peroxidase, also called thyroperoxidase (TPO) or iodide peroxidase, is an enzyme expressed mainly in the thyroid where it is secreted into colloid. Thyroid peroxidase oxidizes iodide ions to form iodine atoms for addition onto tyrosine residues on thyroglobulin for the production of thyroxine (T4) or triiodothyronine (T3), the thyroid hormones. In humans, thyroperoxidase is encoded by the TPO gene. Inorganic iodine enters the body primarily as iodide, I−. After entering the thyroid follicle (or thyroid follicular cell) via a Na+/I− symporter (NIS) on the basolateral side, iodide is shuttled across the apical membrane into the colloid via pendrin, after which thyroid peroxidase oxidizes iodide to atomic iodine (I) or iodinium (I+). The "organification of iodine," the incorporation of iodine into thyroglobulin for the production of thyroid hormone, is nonspecific; that is, there is no TPO-bound intermediate, but iodination occurs via reactive iodine species released from TPO. The chemical reactions catalyzed by thyroid peroxidase occur on the outer apical membrane surface and are mediated by hydrogen peroxide.
背景文献
暂无
亚细胞定位
Membrane.
UNIPROT
别名
MSA antibody
PERT_HUMAN antibody
TDH2A antibody
Thyroid microsomal antigen antibody
Thyroid peroxidase antibody
Thyroperoxidase antibody
TPO antibody
TPX antibody
图片
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Immunohistochemical analysis of paraffin-embedded human follicular thyroid carcinoma tissue using anti-Thyroid Peroxidase(TPO) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM60135, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma tissue using anti-Thyroid Peroxidase(TPO) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM60135, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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