S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
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Catalog# ET1610-3
S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
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WB
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IF-Tissue
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IHC-P
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IHC-Fr
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IF-Cell
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FC
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IP
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Human
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Mouse
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Rat
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Zebrafish
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Goat
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Cynomolgus monkey
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Green monkey
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Pig
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Rabbit
预测的种属支持售后
概述
产品名称
S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within C-terminal human S100 beta.
种属反应性
Human, Mouse, Rat, Zebrafish (Predicted: Goat, Cynomolgus monkey, Green monkey, Pig, Rabbit)
预测的种属支持售后
验证应用
WB, IF-Tissue, IHC-P, IHC-Fr, IF-Cell, FC, IP
分子量
Predicted band size: 11 kDa
阳性对照
Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, mouse liver tissue lysates, SK-MEL-28 cell lysate, Rat brain tissue lysate, zebrafish tissue lysates, A375.
偶联
unconjugated
克隆号
SC57-02
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Tissue
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1:500-1:1,000
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IHC-P
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1:2,000
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IHC-Fr
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1:1,000
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IF-Cell
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1:200
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
发表文章中的应用
| IHC | 查看 2 篇文献如下 |
| IF | 查看 2 篇文献如下 |
发表文章中的种属
| Human | 查看 2 篇文献如下 |
| Mouse | 查看 2 篇文献如下 |
| Rat | 查看 1 篇文献如下 |
靶点
功能
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
背景文献
1. Miyamoto Y et al. Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination. Mol Biol Cell 26:3489-503 (2015).
2. Gondo A et al. Sustained Down-regulation of -Dystroglycan and Associated Dysfunctions of Astrocytic Endfeet in Epileptic Cerebral Cortex. J Biol Chem 289:30279-88 (2014).
序列相似性
Belongs to the S-100 family.
组织特异性
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
亚细胞定位
Cytoplasm, Nucleus.
别名
NEF antibody
Protein S100 B antibody
Protein S100-B antibody
S 100 calcium binding protein beta chain antibody
S 100 protein beta chain antibody
S-100 protein beta chain antibody
S-100 protein subunit beta antibody
S100 antibody
S100 calcium binding protein beta (neural) antibody
S100 calcium-binding protein B antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
Application: IF-tissue
Species: Mouse
Site: Hippocampus
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-3) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of S100 beta on mouse liver tissue lysates with Rabbit anti-S100 beta antibody (ET1610-3) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of S100 beta on different lysates with Rabbit anti-S100 beta antibody (ET1610-3) at 1/2,000 dilution.
Lane 1: SK-MEL-28 cell lysate (15 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-3) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of S100 beta on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Predicted band size: 11 kDa
Observed band size: 14 kDa -
Immunocytochemistry analysis of A375 cells labeling S100 beta with Rabbit anti-S100 beta antibody (ET1610-3) at 1/200 dilution.
Cells were fixed in ice-cold 100% methanol for 5 minutes, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100 beta antibody (ET1610-3) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of A375 cells labeling S100 beta.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1610-3, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Neuronal TRPV1-CGRP axis regulates peripheral nerve regeneration through ERK/HIF-1 signaling pathway
Author: Huiling Che, Yu Du, Yixuan Jiang, Zhanfeng Zhu, Mingxuan Bai, Jianan Zheng, Mao Yang, Lin Xiang, Ping Gong
PMID: 39792906
应用: IF
反应种属: Mouse,Rat
发表时间: 2025 Jan
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Citation
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MSX1+ PDGFRAlow limb mesenchyme-like cells as an efficient stem cell source for human cartilage regeneration
Author: Liao Yuansong,et al
PMID: 38428414
应用: IF
反应种属: Mouse
发表时间: 2024 Mar
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Citation
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Three-Dimensional Cell Spheroid Culture and Proliferation Activity Study of Uveal Melanoma Cell Line C918
Author: Yu Jinhai,et al
PMID: NO PMID20240813
应用: IHC
反应种属: Human
发表时间: 2024 Aug
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Citation
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A single-cell atlas of liver metastases of colorectal cancer reveals reprogramming of the tumor microenvironment in response to preoperative chemotherapy
Author: Che, L. H., Liu, J. W., Huo, J. P., Luo, R., Xu, R. M., He, C., Li, Y. Q., Zhou, A. J., Huang, P., Chen, Y. Y., Ni, W., Zhou, Y. X., Liu, Y. Y., Li, H. Y., Zhou, R., Mo, H., & Li, J. M.
PMID: 34489408
应用: IHC
反应种属: Human
发表时间: 2021 Sep
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Citation