Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]
Catalog# HA721486
Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]
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WB
-
FC
-
Human
概述
产品名称
Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Pumilio 2 aa 1-100.
种属反应性
Human
验证应用
WB, FC
分子量
Predicted band size: 114 kDa
阳性对照
A549 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, U-2 OS.
偶联
unconjugated
克隆号
JE37-76
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
FC
-
1:500-1:1,000
靶点
功能
This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants.
背景文献
1. Elguindy MM et al. NORAD-induced Pumilio phase separation is required for genome stability. Nature. 2021 Jul
2. Silva ILZ et al. Modulation and function of Pumilio proteins in cancer. Semin Cancer Biol. 2022 Nov
亚细胞定位
Cytoplasm, Cytoplasmic granule, perinuclear region.
UNIPROT
别名
FLJ36528 antibody
KIAA0235 antibody
MGC138251 antibody
MGC138253 antibody
PUM 2 antibody
PUM2 antibody
PUM2_HUMAN antibody
PUMH 2 antibody
PUMH2 antibody
Pumilio (Drosphila) homolog 2 antibody
展开图片
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Western blot analysis of Pumilio 2 on different lysates with Rabbit anti-Pumilio 2 antibody (HA721486) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 114 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721486) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of U-2 OS cells labeling Pumilio 2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721486, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"