NMI Recombinant Rabbit Monoclonal Antibody [JE65-78]
Catalog# HA720087
NMI Recombinant Rabbit Monoclonal Antibody [JE65-78]
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WB
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IF-Cell
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IHC-P
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FC
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Human
概述
产品名称
NMI Recombinant Rabbit Monoclonal Antibody [JE65-78]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human NMI aa 1-51/307.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 35 kDa
阳性对照
THP-1 cell lysate, HeLa cell lysate, K-562 cell lysate, human spleen tissue, Hela, SiHa.
偶联
unconjugated
克隆号
JE65-78
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:50
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IHC-P
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1:400
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FC
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1:500-1:1,000
靶点
功能
N-myc-interactor also known as N-myc and STAT interactor is a protein that in humans is encoded by the NMI gene. NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
背景文献
1. Ji D. et. al. NMI promotes cell proliferation through TGFbeta/Smad pathway by upregulating STAT1 in colorectal cancer. J Cell Physiol. 2020 Jan
2. Xu X. et. al. Interferon activates promoter of Nmi gene via interferon regulator factor-1. Mol Cell Biochem. 2018 Apr
亚细胞定位
Nucleus, Secreted, Cytoplasm.
UNIPROT
别名
N myc (and STAT) interactor antibody
N myc and STAT interactor antibody
N myc interactor antibody
NMI antibody
NMYC and STAT interactor antibody
NMYC interactor antibody
图片
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Western blot analysis of NMI on different lysates with Rabbit anti-NMI antibody (HA720087) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 39 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720087) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-NMI antibody (HA720087) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720087) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of Hela cells labeling NMI with Rabbit anti-NMI antibody (HA720087) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NMI antibody (HA720087) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of SiHa cells labeling NMI with Rabbit anti-NMI antibody (HA720087) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NMI antibody (HA720087) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
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