ERK2 Recombinant Mouse Monoclonal Antibody [5-D2-R]
Catalog# HA601202
ERK2 Recombinant Mouse Monoclonal Antibody [5-D2-R]
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WB
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Human
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Mouse
-
Rat
概述
产品名称
ERK2 Recombinant Mouse Monoclonal Antibody [5-D2-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human ERK2 aa aa 200-359.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 41 kDa
阳性对照
HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
偶联
unconjugated
克隆号
5-D2-R
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis.
背景文献
1. Wortzel I et al. The ERK cascade: distinct functions within various subcellular organelles. Genes Cancer 2:195-209 (2011).
2. Ohori M et al. Role of a cysteine residue in the active site of ERK and the MAPKK family. Biochem Biophys Res Commun 353:633-637 (2007).
3. Ohori M et al. Identification of a selective ERK inhibitor and structural determination of the inhibitor-ERK2 complex. Biochem Biophys Res Commun 336:357-363 (2005).
亚细胞定位
Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.
别名
ERK 2 antibody
ERK-2 antibody
ERT1 antibody
Extracellular Signal Regulated Kinase 2 antibody
Extracellular signal-regulated kinase 2 antibody
MAP kinase 1 antibody
MAP kinase 2 antibody
MAP kinase isoform p42 antibody
MAPK 1 antibody
MAPK 2 antibody
展开图片
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Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA601202) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: C6 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601202) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA601202) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate (40 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601202) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA601202) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate (10 µg/Lane)
Lane 2: A549-si ERK2 cell lysate (10 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601202) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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