Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)
-
-
-
-
-
-
2+
Catalog# HA601367
Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)
-
IHC-Fr
-
IHC-P
-
Mouse
-
Rat
-
Cynomolgus monkey
-
Pig
预测的种属支持售后
概述
产品名称
Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within N-terminal human Iba1.
产品特异性
This Rat Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (ET1705-78). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
种属反应性
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
IHC-Fr, IHC-P
分子量
Predicted band size: 17 kDa
阳性对照
Mouse brain tissue, mouse striatum tissue, rat striatum tissue.
偶联
unconjugated
克隆号
JM36-62
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-Fr
-
1:500-1:1,000
-
IHC-P
-
1:100-1:500
靶点
功能
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
背景文献
1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).
2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).
亚细胞定位
Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.
别名
AIF 1 antibody
AIF-1 antibody
Aif1 antibody
AIF1 protein antibody
AIF1_HUMAN antibody
Allograft inflammatory factor 1 antibody
Allograft inflammatory factor 1 splice variant G antibody
allograft inflammatory factor-1 splice variant Hara-1 antibody
balloon angioplasty responsive transcription antibody
BART 1 antibody
展开图片
-
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
-
-
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Iba1 antibody (HA601367) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Iba1 antibody (HA601367) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Iba1 antibody (HA601367) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
iFluor™ 488 Conjugated Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]
Application: IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: iFluor™ 488
Iba1 Rabbit Polyclonal Antibody
Application: IHC-P
Reactivity: Human,Mouse
Conjugate: unconjugated
Iba1 Recombinant Rabbit Monoclonal Antibody [JM36-62]
Application: WB,IHC-P,IHC-Fr,IF-Tissue,IF-Cell,FC,IP,mIHC
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
Iba1 Recombinant Mouse Monoclonal Antibody [8G8]
Application: WB,IHC-P,FC,IP,IHC-Fr,IF-Cell
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
Iba1 Recombinant Antibody [JM36-62] - Guinea pig IgG2 (Chimeric)
Application: IHC-Fr,WB
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
