Dopamine Transporter Recombinant Antibody [PSH10-49] - Rat IgG1 (Chimeric)
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Catalog# HA601404
Dopamine Transporter Recombinant Antibody [PSH10-49] - Rat IgG1 (Chimeric)
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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Human
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Cynomolgus monkey
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Pig
预测的种属支持售后
概述
产品名称
Dopamine Transporter Recombinant Antibody [PSH10-49] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein.
种属反应性
Mouse, Rat (Predicted: Human, Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
IHC-Fr, IHC-P, WB
分子量
Predicted band size: 69 kDa
阳性对照
Mouse striatum tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
偶联
unconjugated
克隆号
PSH10-49
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IHC-P
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1:500-1:2,000
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WB
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1:2,000
靶点
功能
The dopamine transporter (also dopamine active transporter, DAT, SLC6A3) is a membrane-spanning protein that pumps the neurotransmitter dopamine out of the synaptic cleft back into cytosol. In the cytosol, other transporters sequester the dopamine into vesicles for storage and later release. Dopamine reuptake via DAT provides the primary mechanism through which dopamine is cleared from synapses, although there may be an exception in the prefrontal cortex, where evidence points to a possibly larger role of the norepinephrine transporter.DAT is implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder, bipolar disorder, clinical depression, alcoholism, and substance use disorder. The gene that encodes the DAT protein is located on human chromosome 5, consists of 15 coding exons, and is roughly 64 kbp long. Evidence for the associations between DAT and dopamine related disorders has come from a type of genetic polymorphism, known as a VNTR, in the DAT gene (DAT1), which influences the amount of protein expressed.
背景文献
1. Savchenko A et al. Dopamine Transporter Deficient Rodents: Perspectives and Limitations for Neuroscience. Biomolecules. 2023 May
2. Ng J et al. Dopamine Transporter Deficiency Syndrome (DTDS): Expanding the Clinical Phenotype and Precision Medicine Approaches. Cells. 2023 Jun
亚细胞定位
Cell membrane.
别名
DA transporter antibody
DAT 1 antibody
DAT antibody
DAT1 antibody
Dopamine transporter 1 antibody
Dopamine transporter antibody
PKDYS antibody
SC6A3_HUMAN antibody
SLC6A3 antibody
Sodium dependent dopamine transporter antibody
展开图片
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Application: IHC-Fr
Species: Mouse
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: Striatum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Dopamine Transporter antibody (HA601404) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601404) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Dopamine Transporter antibody (HA601404) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601404) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of Dopamine Transporter on different lysates with Rat anti-Dopamine Transporter antibody (HA601404) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 75 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601404) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"