KLF5 Recombinant Rabbit Monoclonal Antibody [PSH15-42]
Catalog# HA723748
KLF5 Recombinant Rabbit Monoclonal Antibody [PSH15-42]
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IF-Cell
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FC
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Human
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Mouse
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Rat
概述
产品名称
KLF5 Recombinant Rabbit Monoclonal Antibody [PSH15-42]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human KLF5 aa 81-380.
种属反应性
Human, Mouse, Rat
验证应用
IF-Cell, FC
分子量
Predicted band size: 51 kDa
阳性对照
HeLa, NIH/3T3, C6.
偶联
unconjugated
克隆号
PSH15-42
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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IF-Cell
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1:2,000
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FC
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1:1,000
靶点
功能
Krueppel-like factor 5 is a protein that in humans is encoded by the KLF5 gene. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.
背景文献
1. Luo Y et al. The roles and regulation of the KLF5 transcription factor in cancers. Cancer Sci. 2021 Jun
2. Kyriazis ID et al. KLF5 Is Induced by FOXO1 and Causes Oxidative Stress and Diabetic Cardiomyopathy. Circ Res. 2021 Feb
亚细胞定位
Nucleus.
别名
Basic transcription element binding protein 2 antibody
Basic transcription element-binding protein 2 antibody
BTE binding protein 2 antibody
BTE-binding protein 2 antibody
BTEB 2 antibody
BTEB2 antibody
CKLF antibody
Colon krueppel like factor antibody
Colon krueppel-like factor antibody
GC box binding protein 2 antibody
展开图片
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Immunocytochemistry analysis of HeLa cells labeling KLF5 with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling KLF5 with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of C6 cells labeling KLF5 with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLF5 antibody (HA723748) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling KLF5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723748, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling KLF5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723748, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of C6 cells labeling KLF5.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA723748, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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