RAR Beta Rabbit Polyclonal Antibody
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5+
Catalog# ER1915-71
RAR Beta Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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FC
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Human
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Mouse
概述
产品名称
RAR Beta Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human RAR Beta 155-250/482
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IF-Cell, IF-Tissue, FC
分子量
50 KDa
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-cell
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1:100
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IF-tissue
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1:100-500
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FC
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1ug/Test
靶点
功能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
背景文献
暂无
亚细胞定位
Isoform Beta-1: Nucleus. Isoform Beta-2: Nucleus. Isoform Beta-4: Cytoplasm.
UNIPROT
别名
HAP antibody
HBV-activated protein antibody
NR1B2 antibody
Nuclear receptor subfamily 1 group B member 2 antibody
RAR B antibody
RAR beta antibody
RAR epsilon antibody
RAR-beta antibody
RAR-epsilon antibody
RARB antibody
展开图片
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Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-RAR Beta (ER1915-71) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD -
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-RAR Beta (ER1915-71) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD -
Sample:
Lane 1: Mouse Eye tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Primary: Anti- RAR Beta (ER1915-71) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (ER1915-71) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (ER1915-71) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (ER1915-71) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (ER1915-71) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RAR Beta) Polyclonal Antibody, Unconjugated (ER1915-71) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (RAR Beta) polyclonal Antibody, Unconjugated (ER1915-71) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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