Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
Catalog# HA722308
Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
-
WB
-
Human
概述
产品名称
Retinoic Acid Receptor beta Recombinant Rabbit Monoclonal Antibody [JE36-74]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Retinoic Acid Receptor beta aa 356-455 / 455.
种属反应性
Human
验证应用
WB
分子量
Predicted band size: 50 kDa
阳性对照
U-87 MG cell lysate, MCF7 cell lysate.
偶联
unconjugated
克隆号
JE36-74
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
靶点
功能
Retinoic acid receptor beta (RAR-beta), also known as NR1B2 (nuclear receptor subfamily 1, group B, member 2) is a nuclear receptor that in humans is encoded by the RARB gene. This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. A deregulation of this gene has also been detected in uterine cervical carcinoma preneoplastic lesions. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined.
背景文献
1. Yago-Ibáñez J et al. Retinoic acid receptor-beta prevents cisplatin-induced proximal tubular cell death. Biochim Biophys Acta Mol Basis Dis. 2020 Jul
2. Ciancia M et al. Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration. Prog Neurobiol. 2022 May
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT
别名
HAP antibody
HBV-activated protein antibody
NR1B2 antibody
Nuclear receptor subfamily 1 group B member 2 antibody
RAR B antibody
RAR beta antibody
RAR epsilon antibody
RAR-beta antibody
RAR-epsilon antibody
RARB antibody
展开图片
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Western blot analysis of Retinoic Acid Receptor beta on different lysates with Rabbit anti-Retinoic Acid Receptor beta antibody (HA722308) at 1/1,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 1 minute 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722308) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"