TPOR Recombinant Rabbit Monoclonal Antibody [JE58-00]
Catalog# HA722068
TPOR Recombinant Rabbit Monoclonal Antibody [JE58-00]
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WB
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FC
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Human
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Mouse
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Rat
概述
产品名称
TPOR Recombinant Rabbit Monoclonal Antibody [JE58-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human TPOR aa 586-635 / 635.
种属反应性
Human, Mouse, Rat
验证应用
WB, FC
分子量
Predicted band size: 71 kDa
阳性对照
Mouse placenta tissue lysate, NIH/3T3 cell lysate, A431 cell lysate, rat spleen tissue lysate, mouse spleen tissue lysate, A431.
偶联
unconjugated
克隆号
JE58-00
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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FC
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1:1,000
靶点
功能
The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin, CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
背景文献
1. Jiang X et al. Novel chemical-structure TPOR agonist, TMEA, promotes megakaryocytes differentiation and thrombopoiesis via mTOR and ERK signalings. Phytomedicine. 2023 Feb
2. Guglielmelli P et al. The MPL mutation. Int Rev Cell Mol Biol. 2021
亚细胞定位
Cell membrane, Golgi apparatus, Cell surface.
别名
C MPL antibody
CD110 antibody
mpl antibody
MPLV antibody
Myeloproliferative leukemia protein antibody
Myeloproliferative leukemia virus oncogene antibody
Proto-oncogene c-Mpl antibody
THCYT2 antibody
Thrombopoietin receptor antibody
TPO R antibody
展开图片
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Western blot analysis of TPOR on different lysates with Rabbit anti-TPOR antibody (HA722068) at 1/1,000 dilution.
Lane 1: Mouse placenta tissue lysate (40 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (no heat) (20 µg/Lane)
Lane 4: Rat spleen tissue lysate (40 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 71 kDa
Observed band size: 80 kDa
Exposure time: Lane 1-3: 24 seconds; Lane 4-5: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of A431 cells labeling TPOR.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722068, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"