F-spondin Recombinant Rabbit Monoclonal Antibody [PSH02-84]
Catalog# HA721863
F-spondin Recombinant Rabbit Monoclonal Antibody [PSH02-84]
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WB
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IF-Cell
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FC
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Human
概述
产品名称
F-spondin Recombinant Rabbit Monoclonal Antibody [PSH02-84]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Spondin-1 201-250 / 807.
种属反应性
Human
验证应用
WB, IF-Cell, FC
分子量
Predicted band size: 91 kDa
阳性对照
SW1990 cell lysate, PANC-1 cell lysate, U-2 OS cell lysate, HepG2 cell lysate, PANC-1, SW1990.
偶联
unconjugated
克隆号
PSH02-84
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:100-1:500
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FC
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1:1,000
靶点
功能
Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
背景文献
1. Miyakawa R, Kobayashi M, Sugimoto K, Endo Y, Kojima M, Kobayashi Y, Furukawa S, Honda T, Watanabe T, Asano S, Soeda S, Hashimoto Y, Fujimori K, Chiba H. SPON1 is an independent prognostic biomarker for ovarian cancer. J Ovarian Res. 2023 May 13;16(1):95.
2. Huo Y, Yang J, Zheng J, Xu D, Yang M, Tao L, Yao H, Fu X, Yang J, Liu D, Hua R, Zhang J, Sun Y, Hu L, Liu W. Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling. Cell Prolif. 2022 May;55(5):e13237.
3. Park SY, Kang JY, Lee T, Nam D, Jeon CJ, Kim JB. SPON1 Can Reduce Amyloid Beta and Reverse Cognitive Impairment and Memory Dysfunction in Alzheimer\'s Disease Mouse Model. Cells. 2020 May 21;9(5):1275.
亚细胞定位
Secreted protein; extracellular space; extracellular matrix.
UNIPROT
别名
F spondin antibody
SPON 1 antibody
spondin 1 (f spondin) extracellular matrix protein antibody
Spondin 1 antibody
Spondin 1 extracellular matrix protein antibody
Spondin 1 precursor antibody
Spondin1 antibody
Vascular smooth muscle cell growth promoting factor antibody
VSGP antibody
VSGP/F spondin antibody
图片
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Western blot analysis of F-spondin on different lysates with Rabbit anti-F-spondin antibody (HA721863) at 1/1,000 dilution.
Lane 1: SW1990 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 91-120 kDa
Exposure time: 1 minutes 17 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721863) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of PANC-1 cells labeling F-spondin with Rabbit anti-F-spondin antibody (HA721863) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-F-spondin antibody (HA721863) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of SW1990 cells labeling F-spondin with Rabbit anti-F-spondin antibody (HA721863) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-F-spondin antibody (HA721863) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of PANC-1 cells labeling F-spondin.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721863, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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