COMP Mouse Monoclonal Antibody [A10H2]
Catalog# HA601354
COMP Mouse Monoclonal Antibody [A10H2]
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WB
-
IHC-P
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Human
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Mouse
-
Rat
概述
产品名称
COMP Mouse Monoclonal Antibody [A10H2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within human COMP aa 321-370.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 83 kDa
阳性对照
Mouse articular cartilage tissue lysate, Rat articular cartilage tissue lysate, HCT 116 cell lysate, mouse cartilage tissue, rat cartilage tissue.
偶联
unconjugated
克隆号
A10H2
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:1,000
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IHC-P
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1:400
靶点
功能
Cartilage oligomeric matrix protein (COMP), also known as thrombospondin-5, is an extracellular matrix (ECM) protein primarily present in cartilage. In humans it is encoded by the COMP gene. The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfide bonds. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). COMP is a marker of cartilage turnover. It is present in high quantities in fibrotic scars and systemic sclerosis, and it appears to have a role in vascular wall remodeling.
背景文献
1. Cai X et al. COMP Improves Ang-II-Induced Atrial Fibrillation via TGF-beta Signaling Pathway. Cardiovasc Toxicol. 2023 Oct
2. Wang Y et al. COMP promotes pancreatic fibrosis by activating pancreatic stellate cells through CD36-ERK/AKT signaling pathways. Cell Signal. 2024 Jun
亚细胞定位
Secreted, extracellular space, extracellular matrix.
别名
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
Cartilage oligomeric matrix protein antibody
Cartilage oligomeric matrix protein precursor antibody
COMP antibody
COMP_HUMAN antibody
EDM 1 antibody
EDM1 antibody
EPD 1 antibody
EPD1 antibody
Epiphyseal dysplasia 1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution.
Lane 1: Mouse articular cartilage tissue lysate (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat articular cartilage tissue lysate (40 µg/Lane)
Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate (15 µg/Lane)
Lane 2: HCT 116 cell lysate (30 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 100 kDa
Exposure time: 3 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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