SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [H4G1] - BSA and Azide free (Capture)
概述
产品名称
SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [H4G1] - BSA and Azide free (Capture)
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419.
种属反应性
SARS-CoV-2
验证应用
WB, ELISA(Cap)
分子量
46 kDa
阳性对照
SARS-CoV-2 nucleocapsid protein.
偶联
unconjugated
克隆号
H4G1
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH 7.4).
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
WB
-
0.5-2ug/ml
-
ELISA(Cap)
-
5ng/ml
靶点
功能
The nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also interacts with the viral membrane protein during viral assembly, assists in RNA synthesis and folding, plays a role in virus budding, and affects host cell responses, including cell cycle and translation. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
背景文献
1. Fan Wu. et. al. A new coronavirus associated with human respiratory disease in China. Nature. 2020 Mar;579(7798):265-269.
亚细胞定位
Virion.
别名
2019-nCoV nucleocapsid protein antibody
N antibody
NC antibody
Nucleoprotein antibody
Protein N antibody
图片
-
Fig1: The binding activity of HA600006 with Recombinant SARS-CoV-2 Nucleocapsid protein.
Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600006) for 1 hour at 25℃. The EC50 of HA600006 is 10.31 ng/ml. -
This antibody will detect SARS-CoV-2 Nucleocapsid protein in ELISA pair set (Cat: # HA600006). In a sandwich ELISA, it can be used as Capture antibody when paired with (Clone: # A4G9-HRP).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"