NF-kB p100 / NFKB2 Mouse Monoclonal Antibody [16B2]
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Catalog# EM1901-78
NF-kB p100 / NFKB2 Mouse Monoclonal Antibody [16B2]
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WB
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IHC-P
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FC
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Human
概述
产品名称
NF-kB p100 / NFKB2 Mouse Monoclonal Antibody [16B2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human NFKB2 aa 1-50 / 900.
产品特异性
NF-κB2 p100 (16B2) Mouse mAb detects endogenous levels of both the p100 precursor and the p52 active form of NFKB2.
种属反应性
Human
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 97 kDa
阳性对照
Human tonsil tissue, human placenta tissue, A431 cell, Siha cell lysates.
偶联
unconjugated
克隆号
16B2
RRID
产品特性
形态
Liquid
浓度
2ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Human | 查看 1 篇文献如下 |
靶点
功能
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor.
背景文献
1. Dobrzanski P. et. al. Differential interactions of Rel-NF-kappa B complexes with I kappa B alpha determine pools of constitutive and inducible NF-kappa B activity. EMBO J. 13:4608-4616(1994)
翻译后修饰
While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.; Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing.; Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT
别名
CVID10 antibody
DNA binding factor KBF2 antibody
H2TF1 antibody
Lymphocyte translocation chromosome 10 protein antibody
LYT 10 antibody
NF kB2 antibody
NFKB p52/p100 subunit antibody
Nuclear factor Kappa B subunit 2 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of NF-kB p100 / NFKB2 on different lysates with Mouse anti-NF-kB p100 / NFKB2 antibody (EM1901-78) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-NF-kB p100 / NFKB2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 120 kDa
Exposure time: 60 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-78) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of NF-kB p100 / NFKB2 on Siha cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Predicted band size: 97 kDa
Observed band size: 52/100 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NF-kB p100 / NFKB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-78, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NF-kB p100 / NFKB2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-78, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of NF-kB p100 / NFKB2 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488 Goat anti-Mouse IgG antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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LTBP2 regulates cisplatin resistance in GC cells via activation of the NF-κB2/BCL3 pathway
Author: Jun Wang , Wenjia Liang , Xiangwen Wang , Zhao Chen , Lei Jiang
PMID: 38577985
应用: WB
反应种属: Human
发表时间: 2024 Apr
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Citation
同靶点 & 同通路的产品
NF-kB p100 / NFKB2 Mouse Monoclonal Antibody [16B3]
Application: WB,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
