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Calreticulin Mouse Monoclonal Antibody [7B1]

RMB: 1500

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Catalog# EM1701-60

Calreticulin Mouse Monoclonal Antibody [7B1]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Calreticulin Mouse Monoclonal Antibody [7B1]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant full length protein corresponding to Human Calretinin aa 1 to the C-terminus.

种属反应性

Human

验证应用

WB, IHC-P, IF-Cell, FC

分子量

Predicted band size: 48 kDa

阳性对照

HepG2 cell lysate, HeLa cell lysate, HL-60 cell lysate, HepG2, human prostate cancer tissue, Jurkat.

偶联

unconjugated

克隆号

7B1

RRID

AB_3068784

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein G affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下

靶点

功能

Calnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

背景文献

1. Nauseef W M et al. Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase. J Biol Chem 270:4741-4747 (1995).

2. Holaska J M et al. Calreticulin is a receptor for nuclear export. J Cell Biol 152:127-140 (2001).

序列相似性

Belongs to the calreticulin family.

亚细胞定位

Endoplasmic reticulum. Secreted. Cytosol.

UNIPROT

SwissProt: P27797 Human

别名

Autoantigen RO antibody

CALR antibody

CALR protein antibody

CALR_HUMAN antibody

Calregulin antibody

Calreticulin antibody

cC1qR antibody

CRP55 antibody

CRT antibody

CRTC antibody

展开

图片

  • Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (<a href="/products/EM1701-60" style="font-weight: bold;text-decoration: underline;">EM1701-60</a>) at 1/5,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: HL-60 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 48 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-60" style="font-weight: bold;text-decoration: underline;">EM1701-60</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (EM1701-60) at 1/5,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: HL-60 cell lysate (20 µg/Lane)

    Predicted band size: 48 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-60) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (<a href="/products/EM1701-60" style="font-weight: bold;text-decoration: underline;">EM1701-60</a>) at 1/500 dilution.<br /><br />Lane 1: Hela-si NT cell lysate (10 µg/Lane)<br />Lane 2: Hela-si Calreticulin cell lysate (10 µg/Lane)<br /><br />Predicted band size: 48 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 5 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br /><a href="/products/EM1701-60" style="font-weight: bold;text-decoration: underline;">EM1701-60</a> was shown to specifically react with Calreticulin in Hela-si NT cells. Weakened band was observed when Hela-si Calreticulin sample was tested. Hela-si NT and Hela-si Calreticulin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-60" style="font-weight: bold;text-decoration: underline;">EM1701-60</a>, 1/500) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-mouse IgG-HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:150,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (EM1701-60) at 1/500 dilution.

    Lane 1: Hela-si NT cell lysate (10 µg/Lane)
    Lane 2: Hela-si Calreticulin cell lysate (10 µg/Lane)

    Predicted band size: 48 kDa
    Observed band size: 55 kDa

    Exposure time: 5 seconds;

    4-20% SDS-PAGE gel.

    EM1701-60 was shown to specifically react with Calreticulin in Hela-si NT cells. Weakened band was observed when Hela-si Calreticulin sample was tested. Hela-si NT and Hela-si Calreticulin samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM1701-60, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-mouse IgG-HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

  • ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.

  • Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

    Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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