Olig2 Recombinant Rabbit Monoclonal Antibody
Catalog# IRS067
Olig2 Recombinant Rabbit Monoclonal Antibody
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mIHC
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Mouse
概述
产品名称
Olig2 Recombinant Rabbit Monoclonal Antibody
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Olig2 aa 238-287 / 323.
种属反应性
Mouse
验证应用
mIHC
分子量
Predicted band size: 32 kDa
阳性对照
Mouse brain tissue.
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
mIHC
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1:100
靶点
功能
The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG2 is more widely expressed in embryonic brain than OLIG1, while OLIG3 is primarily expressed in non-neural tissues. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.
背景文献
1. Kiely AP et al. a-Synucleinopathy associated with G51D SNCA mutation: a link between Parkinson\'s disease and multiple system atrophy Acta Neuropathol 125:753-69 (2013).
2. Wang K et al. Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells. Cloning Stem Cells 11:483-96 (2009).
组织特异性
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT
别名
Basic domain helix loop helix protein class B 1 antibody
Basic helix loop helix protein class B 1 antibody
BHLHB antibody
bHLHB1 antibody
bHLHe19 antibody
Class B basic helix loop helix protein 1 antibody
Class B basic helix-loop-helix protein 1 antibody
class E basic helix loop helix protein 19 antibody
Class E basic helix-loop-helix protein 19 antibody
Human protein kinase C binding protein RACK17 antibody
展开图片
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Olig2 antibody (IRS067) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with SOX2, TBR1 (IRS070), Olig2 (IRS067), CD34 (IRS050) and GFAP (IRS069) antibody at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"