USP13 Rabbit Polyclonal Antibody
Catalog# ER1803-09
USP13 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
FC
-
IHC-P
-
Human
-
Mouse
-
Rat
概述
产品名称
USP13 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within Human USP13 aa 570-776 / 863.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, FC, IHC-P
分子量
97 kDa
阳性对照
293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50-1:200
-
FC
-
1:50-1:100
-
IHC-P
-
1:100-1:200
靶点
功能
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.
背景文献
1. Liu J et al. Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13. Cell 147:223-234 (2011).
2. Scortegagna M et al. USP13 enzyme regulates Siah2 ligase stability and activity via noncatalytic ubiquitin-binding domains. J Biol Chem 286:27333-27341 (2011).
序列相似性
Belongs to the peptidase C19 family.
组织特异性
Highly expressed in ovary and testes.
亚细胞定位
Nucleus. Cytosol.
别名
Deubiquitinating enzyme 13 antibody
Isopeptidase T 3 antibody
Isopeptidase T-3 antibody
Isopeptidase T3 antibody
ISOT 3 antibody
ISOT-3 antibody
ISOT3 antibody
Ubiquitin carboxyl terminal hydrolase 13 antibody
Ubiquitin carboxyl-terminal hydrolase 13 antibody
Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
展开图片
-
☑ Knockdown (KD)
Western blot analysis of USP13 on different lysates with Rabbit anti-USP13 antibody (ER1803-09) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-USP13 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-09) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain -
ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
