USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]
Catalog# ET1706-14
USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]
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WB
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IP
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Human
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Mouse
概述
产品名称
USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human USP13 aa 739-788 / 863.
种属反应性
Human, Mouse
验证应用
WB, IP
分子量
Predicted band size: 97 kDa
阳性对照
SH-SY5Y cell lysates, mouse testis tissue lysates.
偶联
unconjugated
克隆号
JU72-38
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IP
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1:10-1:50
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Mouse | 查看 1 篇文献如下 |
靶点
功能
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.
背景文献
1. Esquerdo M et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS One 11:e0150364 (2016).
2. Geng J et al. Down-regulation of USP13 mediates phenotype transformation of fibroblasts in idiopathic pulmonary fibrosis. Respir Res 16:124 (2015).
序列相似性
Belongs to the peptidase C19 family.
组织特异性
Highly expressed in ovary and testes.
亚细胞定位
Nucleus. Cytoplasm.
别名
Deubiquitinating enzyme 13 antibody
Isopeptidase T 3 antibody
Isopeptidase T-3 antibody
Isopeptidase T3 antibody
ISOT 3 antibody
ISOT-3 antibody
ISOT3 antibody
Ubiquitin carboxyl terminal hydrolase 13 antibody
Ubiquitin carboxyl-terminal hydrolase 13 antibody
Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
展开图片
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Western blot analysis of USP13 on SH-SY5Y cell lysates with Rabbit anti-USP13 antibody (ET1706-14) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of USP13 on mouse testis tissue lysates with Rabbit anti-USP13 antibody (ET1706-14) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-14) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Chloroquine Intervenes Nephrotoxicity of Nilotinib through Deubiquitinase USP13‐Mediated Stabilization of Bcl‐XL
Author:
PMID: 37452432
期刊: Advanced Science
应用: WB
反应种属: Mouse
发表时间: 2023 Jul
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Citation