USP22 Recombinant Rabbit Monoclonal Antibody [JU63-27]
Catalog# ET1706-13
USP22 Recombinant Rabbit Monoclonal Antibody [JU63-27]
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WB
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IHC-P
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IF-Tissue
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IP
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Human
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Mouse
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Rat
概述
产品名称
USP22 Recombinant Rabbit Monoclonal Antibody [JU63-27]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human USP22 aa 321-370 / 525.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue, IP
分子量
Predicted band size: 60 kDa
阳性对照
HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Neuro-2a cell lysate, F9 cell lysate, C6 cell lysate, PC-12 cell lysate, Human brain tissue lysate, Human liver tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
JU63-27
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IHC-P
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1:200
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IF-Tissue
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1:50
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IP
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1-2μg/sample
发表文章中的应用
| WB | 查看 1 篇文献如下 |
| IHC-P | 查看 1 篇文献如下 |
发表文章中的种属
| Human | 查看 1 篇文献如下 |
靶点
功能
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The gene encoding USP22 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.
背景文献
1. Xu G et al. MicroRNA-30e-5p suppresses non-small cell lung cancer tumorigenesis by regulating USP22-mediated Sirt1/JAK/STAT3 signaling. Exp Cell Res pii: S0014-4827(17)30630-4 (2017) .
2. Wang A et al. USP22 Induces Cisplatin Resistance in Lung Adenocarcinoma by Regulating γH2AX-Mediated DNA Damage Repair and Ku70/Bax-Mediated Apoptosis. Front Pharmacol. 8:274 (2017).
序列相似性
Belongs to the peptidase C19 family. UBP8 subfamily.
组织特异性
Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
亚细胞定位
Nucleus.
别名
Deubiquitinating enzyme 22 antibody
KIAA1063 antibody
Ubiquitin carboxyl terminal hydrolase 22 antibody
Ubiquitin carboxyl-terminal hydrolase 22 antibody
Ubiquitin specific peptidase 22 antibody
Ubiquitin specific peptidase 3 like antibody
Ubiquitin specific processing protease 22 antibody
Ubiquitin specific protease 22 antibody
Ubiquitin thioesterase 22 antibody
Ubiquitin thiolesterase 22 antibody
展开图片
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Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: Jurkat cell lysate (15 µg/Lane)
Lane 4: MCF7 cell lysate (15 µg/Lane)
Lane 5: Neuro-2a cell lysate (15 µg/Lane)
Lane 6: F9 cell lysate (15 µg/Lane)
Lane 7: C6 cell lysate (15 µg/Lane)
Lane 8: PC-12 cell lysate (15 µg/Lane)
Lane 9: Human brain tissue lysate (30 µg/Lane)
Lane 10: Human liver tissue lysate (30 µg/Lane)
Lane 11: Mouse brain tissue lysate (30 µg/Lane)
Lane 12: Rat brain tissue lysate (30 µg/Lane)
Lane 13: Rat liver tissue lysate (30 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: Lane 1-3: 6 seconds; Lane 4-13: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate (10 µg/Lane)
Lane 2: HAP1-USP22 KD cell lysate (10 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 60 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-USP22 antibody (ET1706-13) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-USP22 antibody (ET1706-13) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling USP22 with Rabbit anti-USP22 antibody (ET1706-13) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1706-13, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
USP22 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1706-13 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1706-13 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: ET1706-13 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of ET1706-13 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 14 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Intelligent Nano-Cage for Precision Delivery of CRISPR-Cas9 and ACC Inhibitors to Enhance Antitumor Cascade Therapy Through Lipid Metabolism Disruption
Author: Wei Zhao, La Zhang, Jiao Guo, Qing Xu, Mi Zhang, Hongqing Liu, Cong Ren, Yifan Zhao, Jianwei Wang, Qiling Peng, Ning Jiang
PMID: 6NOPMID25031301
期刊: Advanced Functional Materials
应用: WB,IHC-P
反应种属: Human
发表时间: 2025 Jan
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Citation