Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08]
Catalog# ET1610-25
Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08]
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WB
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IHC-P
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IP
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IF-Cell
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Human
概述
产品名称
Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Aurora B aa 1-50 / 344.
种属反应性
Human
验证应用
WB, IHC-P, IP, IF-Cell
分子量
Predicted band size: 39 kDa
阳性对照
Hela cell lysate, MCF-7 cell lysate, human tonsil tissue, HeLa.
偶联
unconjugated
克隆号
SC55-08
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:100
发表文章中的应用
| WB | 查看 2 篇文献如下 |
发表文章中的种属
| Human | 查看 2 篇文献如下 |
靶点
功能
Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.
背景文献
1. Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016).
2. Jiang J et al. Cardiac myosin binding protein C regulates postnatal myocyte cytokinesis. Proc Natl Acad Sci U S A 112:9046-51 (2015).
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
组织特异性
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
翻译后修饰
The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.; Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
亚细胞定位
Nucleus, Cytoplasm, Chromosome.
别名
AIK2 antibody
AIM-1 antibody
AIM1 antibody
ARK-2 antibody
ARK2 antibody
AurB antibody
AURKB antibody
AURKB_HUMAN antibody
Aurora 1 antibody
Aurora and Ipl1 like midbody associated protein 1 antibody
展开图片
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Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela cell lysates
Lane 2: MCF-7 cell lysates -
☑ Knockdown (KD)
All lanes: Western blot analysis of Aurora B with anti-Aurora B antibody (ET1610-25) at 1:500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2: Aurora B knockdown Hela whole cell lysate (10 µg).
ET1610-25 was shown to specifically react with Aurora B in wild-type Hela cells. Weakened band was observed when Aurora B knockdown sample was tested. Wild-type and Aurora B knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-25, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin.
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Immunocytochemistry analysis of HeLa cells labeling Aurora B with Rabbit anti-Aurora B antibody (ET1610-25) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora B antibody (ET1610-25) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Overexpression of Aurora Kinase B Is Correlated with Diagnosis and Poor Prognosis in Hepatocellular Carcinoma
Author: Zhang JIn,et al
PMID: 38396874
期刊: International Journal Of Molecular Sciences
应用: WB
反应种属: Human
发表时间: 2024 Feb
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Citation
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Multi-omics analysis identifies potential mechanisms of AURKB in mediating poor outcome of lung adenocarcinoma.
Author: Huang, J., Zhang, Q., Shen, J., Chen, X., & Ma, S.
PMID: 33612479
期刊: Aging (Albany Ny)
应用: WB
反应种属: Human
发表时间: 2021 Feb
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Citation