YB1 Recombinant Rabbit Monoclonal Antibody [ST0432]
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Catalog# ET1609-10
YB1 Recombinant Rabbit Monoclonal Antibody [ST0432]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
概述
产品名称
YB1 Recombinant Rabbit Monoclonal Antibody [ST0432]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human YB1 aa 275-324 / 324.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
分子量
Predicted band size: 36 kDa
阳性对照
HeLa cell lysate, Jurkat cell lysate, PC-12, human kidney tissue, mouse colon tissue, mouse stomach tissue, human prostate carcinoma tissue, Hela.
偶联
unconjugated
克隆号
ST0432
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
靶点
功能
Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes.
背景文献
1. Satkunanathan S et al. Establishment of a novel cell line for the enhanced production of recombinant adeno-associated virus vectors for gene therapy. Hum Gene Ther 25:929-41 (2014).
2. Imada K et al. Mutual regulation between Raf/MEK/ERK signaling and Y-box-binding protein-1 promotes prostate cancer progression. Clin Cancer Res 19:4638-50 (2013).
序列相似性
Belongs to the YBX1 family.
翻译后修饰
Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability.; In the absence of phosphorylation the protein is retained in the cytoplasm.; Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus (By similarity).
亚细胞定位
Cytoplasm, Nucleus, Cytoplasmic granule, Secreted.
别名
BP 8 antibody
CBF-A antibody
CCAAT binding transcription factor I subunit A antibody
CCAAT-binding transcription factor I subunit A antibody
CSDA2 antibody
CSDB antibody
DBPB antibody
DNA binding protein B antibody
DNA-binding protein B antibody
EFI-A antibody
展开图片
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Western blot analysis of YB1 on different lysates with Rabbit anti-YB1 antibody (ET1609-10) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of PC-12 cells labeling YB1 with Rabbit anti-YB1 antibody (ET1609-10) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-YB1 antibody (ET1609-10) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody at 1/200. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody at 1/200. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody at 1/200. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-YB1 antibody (ET1609-10) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"