Integrin beta 1 Recombinant Rabbit Monoclonal Antibody [SR30-03]
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Catalog# ET1602-10
Integrin beta 1 Recombinant Rabbit Monoclonal Antibody [SR30-03]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
概述
产品名称
Integrin beta 1 Recombinant Rabbit Monoclonal Antibody [SR30-03]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Integrin beta-1 aa 652-701 / 798.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 88 kDa
阳性对照
HeLa cell lysate, A431 cell lysate, human skin tissue, mouse skin tissue, rat skin tissue.
偶联
unconjugated
克隆号
SR30-03
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IHC-P
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1:200-1:1,000
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IF-Tissue
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1:100
靶点
功能
Integrin beta-1 (ITGB1), also known as CD29, is a cell surface receptor that in humans is encoded by the ITGB1 gene. This integrin associates with integrin alpha 1 and integrin alpha 2 to form integrin complexes which function as collagen receptors. It also forms dimers with integrin alpha 3 to form integrin receptors for netrin 1 and reelin. These and other integrin beta 1 complexes have been historically known as very late activation (VLA) antigens. Integrin beta 1 is expressed as at least four different isoforms. In cardiac muscle and skeletal muscle, the integrin beta-1D isoform is specifically expressed, and localizes to costameres, where it aids in the lateral force transmission from the Z-discs to the extracellular matrix. Abnormal levels of integrin beta-1D have been found in limb girdle muscular dystrophy and polyneuropathy.
背景文献
1. Szeto HH et al. Improving mitochondrial bioenergetics under ischemic conditions increases warm ischemia tolerance in the kidney. Am J Physiol Renal Physiol 308:F11-21 (2015).
2. Bayer ML et al. Release of tensile strain on engineered human tendon tissue disturbs cell adhesions, changes matrix architecture, and induces an inflammatory phenotype. PLoS One 9:e86078 (2014).
序列相似性
Belongs to the integrin beta chain family.
组织特异性
Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
翻译后修饰
The cysteine residues are involved in intrachain disulfide bonds.
亚细胞定位
Cell membrane, Cell projection, Cleavage furrow
别名
beta1 integrin antibody
CD29 antibody
Fibronectin receptor subunit beta antibody
FNRB antibody
Glycoprotein IIa antibody
GP IIa antibody
GPIIA antibody
Integrin beta-1 antibody
integrin VLA-4 beta subunit antibody
Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
展开图片
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Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 120-140 kDa
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-10) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/10,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Integrin beta 1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 120-140 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-10) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-10) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-10) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Integrin beta 1 with Rabbit anti-Integrin beta 1 antibody (ET1602-10) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1602-10, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
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