Topoisomerase II alpha Rabbit Polyclonal Antibody
-
-
-
-
-
-
-
-
-
5+
Catalog# ER40124
Topoisomerase II alpha Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
预测的种属支持售后
概述
产品名称
Topoisomerase II alpha Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within C-terminal residues of Topoisomerase II alpha.
种属反应性
Human, Mouse (Predicted: Rat)
预测的种属支持售后
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 174 kDa
阳性对照
Jurkat cell lysate, NIH/3T3 cell lysate, A431 cell lysate, L929 cell lysate, A549 cell lysate, Human liver tissue lysate, HUVEC cell lysate, A549, Hela, A431, NIH/3T3, human tonsil tissue, human colon cancer tissue, human colon cancer tissue, Hela.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500
-
IF-Cell
-
1:200
-
IHC-P
-
1:200
-
FC
-
1:100-1:200
靶点
功能
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes.
背景文献
1. "Use of divalent metal ions in the DNA cleavage reaction of human type II topoisomerases." Deweese J.E., Burch A.M., Burgin A.B., Osheroff N. Biochemistry 48:1862-1869(2009)
2. "The structure of DNA-bound human topoisomerase II alpha: conformational mechanisms for coordinating inter-subunit interactions with DNA cleavage." Wendorff T.J., Schmidt B.H., Heslop P., Austin C.A., Berger J.M. J. Mol. Biol. 424:109-124(2012)
序列相似性
Belongs to the type II topoisomerase family.
翻译后修饰
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
亚细胞定位
Cytoplasm, nucleus.
UNIPROT
别名
alpha isozyme antibody
ATP hydrolyzing DNA topoisomerase II alfa antibody
DNA gyrase antibody
DNA topoisomerase (ATP hydrolyzing) antibody
DNA topoisomerase 2 alpha antibody
DNA topoisomerase 2-alpha antibody
DNA topoisomerase II 170 kD antibody
DNA topoisomerase II alpha isozyme antibody
DNA topoisomerase II antibody
DNA Topoisomerase2 antibody
展开图片
-
Western blot analysis of TOP2A on different lysates using anti-TOP2A antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: A431 cell lysate
Lane 4: L929 cell lysate
Lane 5: A549 cell lysate
Lane 6: Human liver tissue lysate
Lane 7: HUVEC cell lysate -
ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.
-
Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody [SY27-00]
Application: WB,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同靶点 & 同通路的产品
