Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody [SY27-00]
Catalog# ET1607-59
Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody [SY27-00]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
概述
产品名称
Topoisomerase II alpha Recombinant Rabbit Monoclonal Antibody [SY27-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Topoisomerase Ⅱ alphaaa 1,482-1,531 / 1,531.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IP
分子量
Predicted band size: 174 kDa
阳性对照
Rat testis tissue lysate, Mouse testis tissue lysate, NIH/3T3 cell lysate, HT-29 cell lysate, Jurkat cell lysate, human tonsil tissue, human testis tissue, mouse testis tissue, rat testis tissue.
偶联
unconjugated
克隆号
SY27-00
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
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IP
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Use at an assay dependent concentration.
靶点
功能
DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.
背景文献
1. Dykhuizen EC et al. BAF complexes facilitate decatenation of DNA by topoisomerase IIa. Nature 497:624-7 (2013).
2. Taniai E et al. Ochratoxin A induces karyomegaly and cell cycle aberrations in renal tubular cells without relation to induction of oxidative stress responses in rats. Toxicol Lett 224:64-72 (2013).
序列相似性
Belongs to the type II topoisomerase family.
翻译后修饰
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
亚细胞定位
Nucleoplasm, Cytoplasm.
别名
alpha isozyme antibody
ATP hydrolyzing DNA topoisomerase II alfa antibody
DNA gyrase antibody
DNA topoisomerase (ATP hydrolyzing) antibody
DNA topoisomerase 2 alpha antibody
DNA topoisomerase 2-alpha antibody
DNA topoisomerase II 170 kD antibody
DNA topoisomerase II alpha isozyme antibody
DNA topoisomerase II antibody
DNA Topoisomerase2 antibody
展开图片
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Western blot analysis of Topoisomerase II alpha on different lysates with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
Lane 1: Rat testis tissue lysate (40 µg/Lane)
Lane 2: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Predicted band size: 174 kDa
Observed band size: 174 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 5: 18 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-59) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Topoisomerase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Topoisomerase II alpha antibody (ET1607-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"