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TRITC Conjugated Goat Anti-Rabbit IgG H&L polyclonal Antibody

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Catalog# HA1016

TRITC Conjugated Goat Anti-Rabbit IgG H&L polyclonal Antibody

Application

  • IF-Cell

  • IF-Tissue

  • FC

Reactivity

  • Rabbit

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

TRITC Conjugated Goat Anti-Rabbit IgG H&L polyclonal Antibody

抗体类型

Goat Polyclonal Antibody

免疫原

Rabbit IgG (H+L).

产品特异性

To minimize cross-reactivity, the TRITC conjugated goat anti-rabbit IgG (H+L) antibodies have been highly cross-adsorbed against human IgG and Mouse IgG.

种属反应性

Rabbit

验证应用

IF-Cell, IF-Tissue, FC

偶联

TRITC

产品特性

形态

Liquid

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.5%BSA, 40%Glycerol.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • FC

  • 1:50-1:100

靶点

功能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.TRITC(tetraethyl rhodamine isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin.The maximum absorption light wavelength is 550nm, and the maximum emission light wavelength is 620nm, showing orange-red fluorescence. Compared with FITC's emerald green fluorescence, it can be used for double labeling or contrast staining.

背景文献

暂无

图片

  • ICC staining Fascin(<a href="/products/ET1705-18" style="font-weight: bold;text-decoration: underline;">ET1705-18</a>)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&amp;L (<a href="/products/HA1016" style="font-weight: bold;text-decoration: underline;">HA1016</a>) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

    ICC staining Fascin(ET1705-18)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

  • ICC staining SMC3(<a href="/products/ET1703-35" style="font-weight: bold;text-decoration: underline;">ET1703-35</a>)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&amp;L (<a href="/products/HA1016" style="font-weight: bold;text-decoration: underline;">HA1016</a>) (1/100)as used as the secondary antibody. The result showed nuclear staining on Hela cells.

    ICC staining SMC3(ET1703-35)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed nuclear staining on Hela cells.

  • IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized human spleen tissue labeling CD34 with (<a href="/products/ET1606-11" style="font-weight: bold;text-decoration: underline;">ET1606-11</a>) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&amp;L (TRITC) (<a href="/products/HA1016" style="font-weight: bold;text-decoration: underline;">HA1016</a>) secondary antibody at 1/200 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is <a href="/products/HA1016" style="font-weight: bold;text-decoration: underline;">HA1016</a> at 1/200 dilution.

    IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized human spleen tissue labeling CD34 with (ET1606-11) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (TRITC) (HA1016) secondary antibody at 1/200 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/200 dilution.

  • Flow cytometric analysis of Hela cells with SMC3 (<a href="/products/ET1703-35" style="font-weight: bold;text-decoration: underline;">ET1703-35</a>) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is <a href="/products/HA1016" style="font-weight: bold;text-decoration: underline;">HA1016</a> at 1/100 dilution.

    Flow cytometric analysis of Hela cells with SMC3 (ET1703-35) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/100 dilution.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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